diff --git a/docs/SOP_alignment_variant_annotation_ultra2.md b/docs/SOP_alignment_variant_annotation_ultra2.md index 097facbc5b7c9b5ff4c2e0393cf40a3c5ac38d0a..82a70900cc7438e859f3c08a52dfd765646e727f 100644 --- a/docs/SOP_alignment_variant_annotation_ultra2.md +++ b/docs/SOP_alignment_variant_annotation_ultra2.md @@ -82,7 +82,7 @@ A [configuration script](../trio_whole_exome_config.sh) sets environment variabl ## Template for bcbio configuration -Bcbio requires a [template file in YAML format](../trio_whole_exome_bcbio_template.yaml). to define the procedures run in the pipeline. Runs from the Edinburgh CRF have a [slightly different template file](../trio_whole_exome_bcbio_crf_template.yaml) which adds read trimming parameters specific to NextSeq 2000 output. +Bcbio requires a [template file in YAML format](../trio_whole_exome_bcbio_template.yaml). to define the procedures run in the pipeline. ## Output diff --git a/trio_whole_exome_bcbio_crf_template.yaml b/trio_whole_exome_bcbio_crf_template.yaml deleted file mode 100644 index e379642d408852e6aff9309fbc3be6ae0502a6ff..0000000000000000000000000000000000000000 --- a/trio_whole_exome_bcbio_crf_template.yaml +++ /dev/null @@ -1,22 +0,0 @@ -details: -- algorithm: - platform: illumina - quality_format: standard - aligner: bwa - align_split_size: false - trim_reads: fastp - adapters: [nextera2, polyg] - mark_duplicates: true - realign: false - recalibrate: true - effects: vep - effects_transcripts: all - variantcaller: gatk-haplotype - indelcaller: false - remove_lcr: true - tools_on: - - vep_splicesite_annotations - analysis: variant2 - genome_build: hg38 -upload: - dir: /scratch/u035/u035/shared/trio_whole_exome/analysis/output diff --git a/trio_whole_exome_bcbio_template.yaml b/trio_whole_exome_bcbio_template.yaml index f6ebbb44f3e55484bf6eab03decdbf5ecb9263f6..e379642d408852e6aff9309fbc3be6ae0502a6ff 100644 --- a/trio_whole_exome_bcbio_template.yaml +++ b/trio_whole_exome_bcbio_template.yaml @@ -3,6 +3,9 @@ details: platform: illumina quality_format: standard aligner: bwa + align_split_size: false + trim_reads: fastp + adapters: [nextera2, polyg] mark_duplicates: true realign: false recalibrate: true diff --git a/trio_whole_exome_crf_config.sh b/trio_whole_exome_crf_config.sh deleted file mode 100644 index 3b7a89fdf2e885ce50a550b089645f45c9bcd194..0000000000000000000000000000000000000000 --- a/trio_whole_exome_crf_config.sh +++ /dev/null @@ -1,21 +0,0 @@ -#!/usr/bin/bash -# -# Basic configuration options for trio WES pipeline -# - -SCRIPTS=/home/u035/u035/shared/scripts -BCBIO_TEMPLATE=$SCRIPTS/trio_whole_exome_bcbio_crf_template.yaml -TARGET=/home/u035/u035/shared/resources/exome_targets/Twist_Exome_RefSeq_targets_hg38.plus15bp.bed -DOWNLOAD_DIR=/scratch/u035/u035/shared/data -REFERENCE_GENOME=/home/u035/u035/shared/software/bcbio/genomes/Hsapiens/hg38/seq/hg38.fa - -BASE=/scratch/u035/u035/shared/analysis -PARAMS_DIR=$BASE/params -READS_DIR=$BASE/reads -CONFIG_DIR=$BASE/config -WORK_DIR=$BASE/work -OUTPUT_DIR=$BASE/output - -ARCHIVE_DIR=/archive/u035/trio_whole_exome - -export PATH=/home/u035/u035/shared/software/bcbio/tools/bin:$PATH