#!/bin/bash #PBS -l walltime=48:00:00 #PBS -l ncpus=16,mem=8gb #PBS -q sgp #PBS -N trio_whole_exome_bcbio #PBS -j oe # enable running singletons if [ -z $PBS_ARRAY_INDEX ] then if [ -z $INDEX ] then export PBS_ARRAY_INDEX=1 else export PBS_ARRAY_INDEX=$INDEX fi fi # Expects environment variables to be set # PROJECT_ID - e.g. 12345_LastnameFirstname # CONFIG_SH - absolute path to configuration script setting environment variables source $CONFIG_SH FAMILY_ID=`head -n $PBS_ARRAY_INDEX $PARAMS_DIR/$PROJECT_ID.family_ids.txt | tail -n 1` CONFIG_FILE=$CONFIG_DIR/${PROJECT_ID}_${FAMILY_ID}.yaml mkdir -p $WORK_DIR/$FAMILY_ID cd $WORK_DIR/$FAMILY_ID bcbio_nextgen.py $CONFIG_FILE -n 16 -t local DATE=$(basename `tail log/bcbio-nextgen.log | grep 'Storing in local filesystem' | tail -n 1 | awk '{ print $6 }' | perl -pe "s/_${PROJECT_ID}.+//"`) if [ -e $OUTPUT_DIR/${DATE}_${PROJECT_ID}_${FAMILY_ID} ] then for INDV in `cut -f 2 $PARAMS_DIR/${PROJECT_ID}_${FAMILY_ID}.ped` do mv $OUTPUT_DIR/$INDV $OUTPUT_DIR/${DATE}_${PROJECT_ID}_${FAMILY_ID}/ done # fix VCF output file names cd $OUTPUT_DIR/${DATE}_${PROJECT_ID}_${FAMILY_ID} if [ -e ${PROJECT_ID}${FAMILY_ID}-gatk-haplotype-annotated.vcf.gz ] then mv ${PROJECT_ID}${FAMILY_ID}-gatk-haplotype-annotated.vcf.gz ${PROJECT_ID}_${FAMILY_ID}-gatk-haplotype-annotated.vcf.gz mv ${PROJECT_ID}${FAMILY_ID}-gatk-haplotype-annotated.vcf.gz.tbi ${PROJECT_ID}_${FAMILY_ID}-gatk-haplotype-annotated.vcf.gz.tbi fi else echo $OUTPUT_DIR/${DATE}_${PROJECT_ID}_${FAMILY_ID} does not exist. fi