diff --git a/examples/extraction/pairs_data/pos010_trap001_tp0001_Brightfield.png b/examples/extraction/pairs_data/pos010_trap001_tp0001_Brightfield.png deleted file mode 100644 index d50e92fbf3a744f01fd44993720c2b2d38b3fb0b..0000000000000000000000000000000000000000 Binary files a/examples/extraction/pairs_data/pos010_trap001_tp0001_Brightfield.png and /dev/null differ diff --git a/examples/extraction/pairs_data/pos010_trap001_tp0001_GFP.png b/examples/extraction/pairs_data/pos010_trap001_tp0001_GFP.png deleted file mode 100644 index 2b50663f26ed9c7f0305c35339d23fbbc1b529d8..0000000000000000000000000000000000000000 Binary files a/examples/extraction/pairs_data/pos010_trap001_tp0001_GFP.png and /dev/null differ diff --git a/examples/extraction/pairs_data/pos010_trap001_tp0001_segoutlines.png b/examples/extraction/pairs_data/pos010_trap001_tp0001_segoutlines.png deleted file mode 100644 index eb29a60c11d99411cad09711b046d1cb32318e4d..0000000000000000000000000000000000000000 Binary files a/examples/extraction/pairs_data/pos010_trap001_tp0001_segoutlines.png and /dev/null differ diff --git a/examples/extraction/pairs_data/pos010_trap050_tp0001_Brightfield.png b/examples/extraction/pairs_data/pos010_trap050_tp0001_Brightfield.png deleted file mode 100644 index 9f26817ea8dffffd33f360dd39031ee59830d673..0000000000000000000000000000000000000000 Binary files a/examples/extraction/pairs_data/pos010_trap050_tp0001_Brightfield.png and /dev/null differ diff --git a/examples/extraction/pairs_data/pos010_trap050_tp0001_GFP.png b/examples/extraction/pairs_data/pos010_trap050_tp0001_GFP.png deleted file mode 100644 index a600fefdf7dff067a3274f808d359491babca203..0000000000000000000000000000000000000000 Binary files a/examples/extraction/pairs_data/pos010_trap050_tp0001_GFP.png and /dev/null differ diff --git a/examples/extraction/pairs_data/pos010_trap050_tp0001_segoutlines.png b/examples/extraction/pairs_data/pos010_trap050_tp0001_segoutlines.png deleted file mode 100644 index 8dc7a456ee8df34e09e6bee62008ecb5653ff24f..0000000000000000000000000000000000000000 Binary files a/examples/extraction/pairs_data/pos010_trap050_tp0001_segoutlines.png and /dev/null differ diff --git a/examples/extraction/pairs_data/pos010_trap081_tp0001_Brightfield.png b/examples/extraction/pairs_data/pos010_trap081_tp0001_Brightfield.png deleted file mode 100644 index 5670a02a388cd94fdbcf9974295b766caa09632c..0000000000000000000000000000000000000000 Binary files a/examples/extraction/pairs_data/pos010_trap081_tp0001_Brightfield.png and /dev/null differ diff --git a/examples/extraction/pairs_data/pos010_trap081_tp0001_GFP.png b/examples/extraction/pairs_data/pos010_trap081_tp0001_GFP.png deleted file mode 100644 index 7b5702fc7a99653930bd22aa3ef520ac2c2749f3..0000000000000000000000000000000000000000 Binary files a/examples/extraction/pairs_data/pos010_trap081_tp0001_GFP.png and /dev/null differ diff --git a/examples/extraction/pairs_data/pos010_trap081_tp0001_segoutlines.png b/examples/extraction/pairs_data/pos010_trap081_tp0001_segoutlines.png deleted file mode 100644 index 909cfe00ec9dedd03e2f8bd565eaa2c18f6a5e02..0000000000000000000000000000000000000000 Binary files a/examples/extraction/pairs_data/pos010_trap081_tp0001_segoutlines.png and /dev/null differ diff --git a/examples/extraction/pos_example.py b/examples/extraction/pos_example.py deleted file mode 100644 index da44f464b54d81dbba3f5902125d1f7b1fe4abc9..0000000000000000000000000000000000000000 --- a/examples/extraction/pos_example.py +++ /dev/null @@ -1,17 +0,0 @@ -from extraction.core.extractor import Extractor -from extraction.core.parameters import Parameters - -params = Parameters( - tree={ - "general": {"None": ["area"]}, - "GFPFast": {"np_max": ["mean", "median", "imBackground"]}, - "pHluorin405": {"np_max": ["mean", "median", "imBackground"]}, - "mCherry": { - "np_max": ["mean", "median", "imBackground", "max5px", "max2p5pc"] - }, - } -) - - -ext = Extractor(params, omero_id=19310) -d = ext.extract_tp(tp=1, tile_size=117) diff --git a/examples/extraction/tiler_error.py b/examples/extraction/tiler_error.py deleted file mode 100644 index 78619c8f5fd4111d412ea0b9962a56049c6586e4..0000000000000000000000000000000000000000 --- a/examples/extraction/tiler_error.py +++ /dev/null @@ -1,39 +0,0 @@ -import matplotlib.pyplot as plt -from core.experiment import Experiment -from core.segment import Tiler - -expt = Experiment.from_source( - 19310, # Experiment ID on OMERO - "upload", # OMERO Username - "***REMOVED***", # OMERO Password - "islay.bio.ed.ac.uk", # OMERO host - port=4064, # This is default -) - - -# Load whole position -img = expt[0, 0, :, :, 2] -plt.imshow(img[0, 0, ..., 0]) -plt.show() - -# Manually get template -tilesize = 117 -x0 = 827 -y0 = 632 -trap_template = img[0, 0, x0 : x0 + tilesize, y0 : y0 + tilesize, 0] -plt.imshow(trap_template) -plt.show() - -tiler = Tiler(expt, template=trap_template) - -# Load images (takes about 5 mins) -trap_tps = tiler.get_tiles_timepoint(0, tile_size=117, z=[2]) - -# Plot found traps -nrows, ncols = (5, 5) -fig, axes = plt.subplots(nrows, ncols) -for i in range(nrows): - for j in range(ncols): - if i * nrows + j < trap_tps.shape[0]: - axes[i, j].imshow(trap_tps[i * nrows + j, 0, 0, ..., 0]) -plt.show() diff --git a/examples/logfile_parser/cExperiment_log_001.txt b/examples/logfile_parser/cExperiment_log_001.txt deleted file mode 100644 index 8db3a2e4b899c5ee597f79569caa8a531607a9ea..0000000000000000000000000000000000000000 --- a/examples/logfile_parser/cExperiment_log_001.txt +++ /dev/null @@ -1,276 +0,0 @@ - - -===================== -06-Jan-2020 18:30:59 Start creating new experiment using parameters: -Omero experiment name: 001 -Temporary working directory: C:06-Jan-2020 18:30:59 Processing position 2 (1108_002) -06-Jan-2020 18:31:00 Processing position 3 (1108_003) -06-Jan-2020 18:31:01 Processing position 4 (1109_004) -06-Jan-2020 18:31:02 Processing position 5 (1109_005) -06-Jan-2020 18:31:04 Processing position 6 (1109_006) -06-Jan-2020 18:31:05 Processing position 7 (1110_007) -06-Jan-2020 18:31:06 Processing position 8 (1110_008) -06-Jan-2020 18:31:07 Processing position 9 (1110_009) -06-Jan-2020 18:31:10 Successfully completed creating new experiment in 11 secs. ---------------------- - - -===================== -06-Jan-2020 18:31:33 Start selecting traps... -06-Jan-2020 18:31:33 Processing position 1 (1108_001) -06-Jan-2020 18:31:40 Remove trap at 550 1188 -06-Jan-2020 18:31:40 Remove trap at 733 1179 -06-Jan-2020 18:31:41 Remove trap at 384 1189 -06-Jan-2020 18:31:42 Remove trap at 201 1186 -06-Jan-2020 18:31:47 Processing position 2 (1108_002) -06-Jan-2020 18:31:52 Remove trap at 384 1060 -06-Jan-2020 18:31:54 Remove trap at 1081 571 -06-Jan-2020 18:32:01 Processing position 3 (1108_003) -06-Jan-2020 18:32:05 Remove trap at 948 1140 -06-Jan-2020 18:32:06 Remove trap at 1141 1174 -06-Jan-2020 18:32:17 Remove trap at 139 1111 -06-Jan-2020 18:32:18 Add trap at 130 1138 -06-Jan-2020 18:32:26 Processing position 4 (1109_004) -06-Jan-2020 18:32:32 Remove trap at 1176 388 -06-Jan-2020 18:32:39 Processing position 5 (1109_005) -06-Jan-2020 18:32:44 Remove trap at 1141 1135 -06-Jan-2020 18:32:51 Remove trap at 955 379 -06-Jan-2020 18:32:55 Processing position 6 (1109_006) -06-Jan-2020 18:33:00 Remove trap at 676 1177 -06-Jan-2020 18:33:01 Remove trap at 1111 1147 -06-Jan-2020 18:33:14 Processing position 7 (1110_007) -06-Jan-2020 18:33:20 Remove trap at 46 46 -06-Jan-2020 18:33:28 Remove trap at 1150 84 -06-Jan-2020 18:33:34 Processing position 8 (1110_008) -06-Jan-2020 18:33:49 Processing position 9 (1110_009) -06-Jan-2020 18:33:55 Add trap at 1153 1129 -06-Jan-2020 18:33:57 Remove trap at 1135 1141 -06-Jan-2020 18:33:57 Remove trap at 1176 1095 -06-Jan-2020 18:34:15 Successfully completed selecting traps in 2.7 mins. ---------------------- - - -===================== -06-Jan-2020 18:34:28 Start setting extraction parameters using parameters: -extractionParameters: { - extractFunction: extractCellDataStandardParfor - functionParameters: { - type: max - channels: 2 3 - nuclearMarkerChannel: NaN - maxPixOverlap: 5 - maxAllowedOverlap: 25 - } -} -06-Jan-2020 18:34:28 Processing position 1 (1108_001) -06-Jan-2020 18:34:28 Processing position 2 (1108_002) -06-Jan-2020 18:34:29 Processing position 3 (1108_003) -06-Jan-2020 18:34:29 Processing position 4 (1109_004) -06-Jan-2020 18:34:30 Processing position 5 (1109_005) -06-Jan-2020 18:34:30 Processing position 6 (1109_006) -06-Jan-2020 18:34:30 Processing position 7 (1110_007) -06-Jan-2020 18:34:31 Processing position 8 (1110_008) -06-Jan-2020 18:34:31 Processing position 9 (1110_009) -06-Jan-2020 18:34:33 Successfully completed setting extraction parameters in 5 secs. ---------------------- - - -===================== -07-Jan-2020 13:17:43 Start tracking traps in time... -07-Jan-2020 13:17:43 Processing position 1 (1108_001) -07-Jan-2020 13:23:31 Processing position 2 (1108_002) -07-Jan-2020 13:29:21 Processing position 3 (1108_003) -07-Jan-2020 13:35:13 Processing position 4 (1109_004) -07-Jan-2020 13:41:19 Processing position 5 (1109_005) -07-Jan-2020 13:47:09 Processing position 6 (1109_006) -07-Jan-2020 13:52:57 Processing position 7 (1110_007) -07-Jan-2020 13:58:41 Processing position 8 (1110_008) -07-Jan-2020 14:04:41 Processing position 9 (1110_009) -07-Jan-2020 14:10:38 Successfully completed tracking traps in time in 52.9 mins. ---------------------- - - -===================== -07-Jan-2020 14:10:38 Start baby segmentation... -07-Jan-2020 14:10:39 Processing position 1 (1108_001) -07-Jan-2020 14:14:32 cTimelapse: 210.344 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 14:18:30 cTimelapse: 240.345 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 14:22:31 cTimelapse: 272.459 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 14:26:32 cTimelapse: 303.876 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 14:30:34 cTimelapse: 336.470 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 14:32:32 Processing position 2 (1108_002) -07-Jan-2020 14:36:22 cTimelapse: 206.699 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 14:40:12 cTimelapse: 235.726 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 14:44:13 cTimelapse: 268.814 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 14:48:27 cTimelapse: 306.046 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 14:52:43 cTimelapse: 343.681 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 14:54:44 Processing position 3 (1108_003) -07-Jan-2020 14:58:47 cTimelapse: 214.895 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 15:02:44 cTimelapse: 247.137 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 15:06:47 cTimelapse: 280.902 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 15:10:51 cTimelapse: 314.796 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 15:15:13 cTimelapse: 354.774 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 15:17:16 Processing position 4 (1109_004) -07-Jan-2020 15:21:06 cTimelapse: 222.663 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 15:25:09 cTimelapse: 253.596 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 15:29:16 cTimelapse: 286.597 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 15:33:46 cTimelapse: 325.040 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 15:38:35 cTimelapse: 369.190 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 15:40:50 Processing position 5 (1109_005) -07-Jan-2020 15:45:01 cTimelapse: 235.110 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 15:49:23 cTimelapse: 268.760 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 15:53:50 cTimelapse: 304.703 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 15:58:15 cTimelapse: 339.861 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 16:02:47 cTimelapse: 377.877 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 16:04:53 Processing position 6 (1109_006) -07-Jan-2020 16:08:32 cTimelapse: 205.246 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 16:12:09 cTimelapse: 231.500 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 16:15:49 cTimelapse: 259.276 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 16:19:45 cTimelapse: 291.813 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 16:24:03 cTimelapse: 331.193 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 16:26:11 Processing position 7 (1110_007) -07-Jan-2020 16:29:30 cTimelapse: 222.990 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 16:32:46 cTimelapse: 238.288 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 16:36:03 cTimelapse: 255.524 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 16:39:21 cTimelapse: 275.165 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 16:42:40 cTimelapse: 297.244 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 16:44:14 Processing position 8 (1110_008) -07-Jan-2020 16:47:32 cTimelapse: 215.583 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 16:50:51 cTimelapse: 235.959 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 16:54:09 cTimelapse: 256.409 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 16:57:25 cTimelapse: 275.563 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 17:00:43 cTimelapse: 296.390 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 17:02:13 Processing position 9 (1110_009) -07-Jan-2020 17:05:35 cTimelapse: 225.847 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 17:08:54 cTimelapse: 245.291 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 17:12:17 cTimelapse: 266.060 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 17:15:41 cTimelapse: 288.448 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 17:19:04 cTimelapse: 311.290 MB; posOverviewGUI: 741.580 MB - -07-Jan-2020 17:20:36 Successfully completed baby segmentation in 3.2 hours. ---------------------- - - -===================== -07-Jan-2020 17:20:37 Start tracking cells using parameters: -Tracking threshold: 10 -07-Jan-2020 17:20:39 Processing position 1 (1108_001) -07-Jan-2020 17:21:23 Processing position 2 (1108_002) -07-Jan-2020 17:22:06 Processing position 3 (1108_003) -07-Jan-2020 17:22:49 Processing position 4 (1109_004) -07-Jan-2020 17:23:33 Processing position 5 (1109_005) -07-Jan-2020 17:24:18 Processing position 6 (1109_006) -07-Jan-2020 17:24:58 Processing position 7 (1110_007) -07-Jan-2020 17:25:25 Processing position 8 (1110_008) -07-Jan-2020 17:25:55 Processing position 9 (1110_009) -07-Jan-2020 17:26:25 Successfully completed tracking cells in 5.8 mins. ---------------------- - - -===================== -07-Jan-2020 17:26:25 Start autoselecting cells using parameters: -Fraction of timelapse that cells are present for: 0.5 -Number of frames a cell must be present: 540 -Cell must appear by frame: 540 -Cell must still be present by frame: 1 -Maximum number of cells: Inf -07-Jan-2020 17:26:27 Processing position 1 (1108_001) -07-Jan-2020 17:26:42 Processing position 2 (1108_002) -07-Jan-2020 17:26:58 Processing position 3 (1108_003) -07-Jan-2020 17:27:14 Processing position 4 (1109_004) -07-Jan-2020 17:27:31 Processing position 5 (1109_005) -07-Jan-2020 17:27:48 Processing position 6 (1109_006) -07-Jan-2020 17:28:03 Processing position 7 (1110_007) -07-Jan-2020 17:28:13 Processing position 8 (1110_008) -07-Jan-2020 17:28:25 Processing position 9 (1110_009) -07-Jan-2020 17:28:36 Successfully completed autoselecting cells in 2.2 mins. ---------------------- - - -===================== -07-Jan-2020 17:28:37 Start extracting cell information... -07-Jan-2020 17:28:39 Processing position 1 (1108_001) -07-Jan-2020 17:58:38 Processing position 2 (1108_002) -07-Jan-2020 18:28:43 Processing position 3 (1108_003) -07-Jan-2020 18:58:45 Processing position 4 (1109_004) -07-Jan-2020 19:29:03 Processing position 5 (1109_005) -07-Jan-2020 19:59:31 Processing position 6 (1109_006) -07-Jan-2020 20:29:01 Processing position 7 (1110_007) -07-Jan-2020 20:56:05 Processing position 8 (1110_008) -07-Jan-2020 21:23:53 Processing position 9 (1110_009) -07-Jan-2020 21:51:15 Successfully completed extracting cell information in 4.4 hours. ---------------------- - - -===================== -07-Jan-2020 21:51:16 Start baby lineage extraction... -07-Jan-2020 21:51:18 Processing position 1 (1108_001) -07-Jan-2020 21:52:37 Processing position 2 (1108_002) -07-Jan-2020 21:53:57 Processing position 3 (1108_003) -07-Jan-2020 21:55:16 Processing position 4 (1109_004) -07-Jan-2020 21:56:36 Processing position 5 (1109_005) -07-Jan-2020 21:57:59 Processing position 6 (1109_006) -07-Jan-2020 21:59:08 Processing position 7 (1110_007) -07-Jan-2020 21:59:50 Processing position 8 (1110_008) -07-Jan-2020 22:00:41 Processing position 9 (1110_009) -07-Jan-2020 22:01:26 Successfully completed baby lineage extraction in 10.2 mins. ---------------------- - - -===================== -07-Jan-2020 22:01:26 Start compiling cell information... -07-Jan-2020 22:01:28 Processing position 1 (1108_001) -07-Jan-2020 22:01:30 Processing position 2 (1108_002) -07-Jan-2020 22:01:33 Processing position 3 (1108_003) -07-Jan-2020 22:01:35 Processing position 4 (1109_004) -07-Jan-2020 22:01:38 Processing position 5 (1109_005) -07-Jan-2020 22:01:40 Processing position 6 (1109_006) -07-Jan-2020 22:01:42 Processing position 7 (1110_007) -07-Jan-2020 22:01:44 Processing position 8 (1110_008) -07-Jan-2020 22:01:46 Processing position 9 (1110_009) -07-Jan-2020 22:02:20 Successfully completed compiling cell information in 54 secs. ---------------------- diff --git a/examples/logfile_parser/example_multiDGUI_acq.txt b/examples/logfile_parser/example_multiDGUI_acq.txt deleted file mode 100644 index e2d1e9b462c12a5f21878b6dd3b6738df7e9e51b..0000000000000000000000000000000000000000 --- a/examples/logfile_parser/example_multiDGUI_acq.txt +++ /dev/null @@ -1,46 +0,0 @@ -Channels: -Channel name, Exposure time, Skip, Z sect., Start time, Camera mode, EM gain, Voltage - Brightfield, 30, 1, 1, 1, 2, 270, 1.000 - GFPFast, 30, 1, 1, 1, 2, 270, 3.500 - mCherry, 100, 1, 1, 1, 2, 270, 2.500 -Z_sectioning: -Sections,Spacing,PFSon?,AnyZ?,Drift,Method - 3, 0.80, 1, 1, 0, 2 -Time_settings: -1,120,660,79200 -Points: -Position name, X position, Y position, Z position, PFS offset, Group, Brightfield, GFPFast, mCherry - pos001, 568.00, 1302.00, 1876.500, 122.450, 1, 30, 30, 100 - pos002, 1267.00, 1302.00, 1880.125, 119.950, 1, 30, 30, 100 - pos003, 1026.00, 977.00, 1877.575, 120.100, 1, 30, 30, 100 - pos004, 540.00, -347.00, 1868.725, 121.200, 2, 30, 30, 100 - pos005, 510.00, -687.00, 1867.150, 122.900, 2, 30, 30, 100 - pos006, -187.00, -470.00, 1864.050, 119.600, 2, 30, 30, 100 - pos007, -731.00, 916.00, 1867.050, 117.050, 3, 30, 30, 100 - pos008, -1003.00, 1178.00, 1866.425, 121.700, 3, 30, 30, 100 - pos009, -568.00, 1157.00, 1868.450, 119.350, 3, 30, 30, 100 - -Flow_control: -Syringe pump details: 2 pumps. -Pump states at beginning of experiment: -Pump port, Diameter, Current rate, Direction, Running, Contents - COM7, 14.43, 0.00, INF, 1, 2% glucose in SC - COM8, 14.43, 4.00, INF, 1, 0.1% glucose in SC - -Dynamic flow details: -Number of pump changes: -1 -Switching parameters: -Infuse/withdraw volumes: -50 -Infuse/withdraw rates: -100 -Times: -0 -Switched to: -2 -Switched from: -1 -Flow post switch: -0 -4 diff --git a/examples/logfile_parser/example_multiDGUI_log.txt b/examples/logfile_parser/example_multiDGUI_log.txt deleted file mode 100644 index e4a242ca9bc785e92a432b8b7965b7e0e581a6dc..0000000000000000000000000000000000000000 --- a/examples/logfile_parser/example_multiDGUI_log.txt +++ /dev/null @@ -1,651 +0,0 @@ -Swain Lab microscope experiment log file -Swain lab microscope control software - MultiDGUI commit number:05903fb3769ccf612e7801b46e2248644ce7ca28 -Warning! - there are uncommitted changes - software may be different from the above commit -29-Feb-2020 -Microscope name is: Batman -Acquisition settings are saved in: -C:\path\to\example_multiDGUI_log.txt -Experiment details: -Aim: Strain: Comments: -Microscope setup for used channels: -Brightfield: -White LED -->(Polariser + Prism + condenser)] -->Filter block:[Dual GFP/mCherry exciter (59022x),Dual dichroic (89021bs),No emission filter] -->Emission filter wheel:[No filter in emission wheel] -GFPFast: -470nm LED -->Combiner cube:[480/40 exciter, 515LP dichroic->(455LP dichroic)] -->Filter block:[Dual GFP/mCherry exciter (59022x),Dual dichroic (89021bs),No emission filter] -->Emission filter wheel:[520/40 emission filter] -mCherry: -White LED -->Combiner cube:[No exciter, No reflecting dichroic->(515LP and 455LP dichroics)] -->Filter block:[Dual GFP/mCherry exciter (59022x),Dual dichroic (89021bs),No emission filter] -->Emission filter wheel:[632/60 emission filter] -Micromanager config file:C:\path\to\config_file.cfg -Omero project: -SteadystateGlucose -Omero tags: -29-Feb-2020,Batman,3 chamber,GFP,mCherry,1106.Mig2-GFP Mig1-mCherry,900.Mig1-GFP Msn2-mCherry,898.Msn2-GFP Mig1-mCherry,0.1% glucose,2% glucose, -Experiment started at:29-Feb-2020 01:16:51 - -------Time point_1------ -Memory available for all arrays: 14141227008 -Memory used by Matlab: 2237132800 -Switching pumps at 29-Feb-2020 01:16:52. Fast infusion/withdrawal step: Rate=100. Volume=50 -Pump switch complete at:29-Feb-2020 01:16:52 -Dominant pump is now: COM8 pumping at 4ul/min -Dominant medium is now: 0.1% glucose in SC -Position:1,pos001 -Position group:1 -Channel configuration set to: Brightfield: 29-Feb-2020 01:16:52 -Moved to X:568, Y:1302 -Call to readDrift after moving to position1 -Setting PFS offset to :122.45 -readDrift script. Reference z position is:1876.5 -readDrift script. Cumulative drift is:-5.9 -Channel configuration set to: Brightfield: 29-Feb-2020 01:17:01 -Channel:Brightfield set at:29-Feb-2020 01:17:01 -Exposure time:30ms -Channel configuration set to: GFPFast: 29-Feb-2020 01:17:06 -Channel:GFPFast set at:29-Feb-2020 01:17:06 -Exposure time:30ms -Exposure time:30ms -captureStack (Batman). Z drive position before stack capture is: 1870.575. 29-Feb-2020 01:17:10 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:17:10 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1870.575. 29-Feb-2020 01:17:10 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:17:10 -Channel configuration set to: mCherry: 29-Feb-2020 01:17:12 -Channel:mCherry set at:29-Feb-2020 01:17:12 -Exposure time:100ms -Exposure time:100ms -captureStack (Batman). Z drive position before stack capture is: 1870.575. 29-Feb-2020 01:17:12 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:17:12 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1870.55. 29-Feb-2020 01:17:13 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:17:13 -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Position:2,pos002 -Position group:1 -Channel configuration set to: Brightfield: 29-Feb-2020 01:17:18 -Moved to X:1267, Y:1302 -Call to readDrift after moving to position2 -Setting PFS offset to :119.95 -readDrift script. Reference z position is:1880.125 -readDrift script. Cumulative drift is:-9.725 -Channel configuration set to: Brightfield: 29-Feb-2020 01:17:27 -Channel:Brightfield set at:29-Feb-2020 01:17:27 -Exposure time:30ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: GFPFast: 29-Feb-2020 01:17:32 -Channel:GFPFast set at:29-Feb-2020 01:17:32 -Exposure time:30ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: mCherry: 29-Feb-2020 01:17:37 -Channel:mCherry set at:29-Feb-2020 01:17:38 -Exposure time:100ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Position:3,pos003 -Position group:1 -Channel configuration set to: Brightfield: 29-Feb-2020 01:17:43 -Moved to X:1026, Y:977 -Call to readDrift after moving to position3 -Setting PFS offset to :120.1 -readDrift script. Reference z position is:1877.575 -readDrift script. Cumulative drift is:-5.925 -Channel configuration set to: Brightfield: 29-Feb-2020 01:17:52 -Channel:Brightfield set at:29-Feb-2020 01:17:52 -Exposure time:30ms -Exposure time:30ms -captureStack (Batman). Z drive position before stack capture is: 1870.925. 29-Feb-2020 01:17:57 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:17:57 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1870.9. 29-Feb-2020 01:17:58 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:17:58 -Channel configuration set to: GFPFast: 29-Feb-2020 01:18:00 -Channel:GFPFast set at:29-Feb-2020 01:18:00 -Exposure time:30ms -Exposure time:30ms -captureStack (Batman). Z drive position before stack capture is: 1870.925. 29-Feb-2020 01:18:01 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:18:01 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1870.925. 29-Feb-2020 01:18:01 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:18:01 -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: mCherry: 29-Feb-2020 01:18:06 -Channel:mCherry set at:29-Feb-2020 01:18:07 -Exposure time:100ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Position:4,pos004 -Position group:2 -Channel configuration set to: Brightfield: 29-Feb-2020 01:18:14 -Moved to X:540, Y:-347 -Call to readDrift after moving to position4 -Setting PFS offset to :121.2 -readDrift script. Reference z position is:1868.725 -readDrift script. Cumulative drift is:3.65 -PFS not locked. Waiting for lock... -PFS took 0.650000 seconds to lock. -Channel configuration set to: Brightfield: 29-Feb-2020 01:18:18 -Channel:Brightfield set at:29-Feb-2020 01:18:18 -Exposure time:30ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: GFPFast: 29-Feb-2020 01:18:23 -Channel:GFPFast set at:29-Feb-2020 01:18:23 -Exposure time:30ms -Exposure time:30ms -captureStack (Batman). Z drive position before stack capture is: 1862.575. 29-Feb-2020 01:18:28 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:18:28 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1862.55. 29-Feb-2020 01:18:28 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:18:28 -Channel configuration set to: mCherry: 29-Feb-2020 01:18:31 -Channel:mCherry set at:29-Feb-2020 01:18:31 -Exposure time:100ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Position:5,pos005 -Position group:2 -Channel configuration set to: Brightfield: 29-Feb-2020 01:18:37 -Moved to X:510, Y:-687 -Call to readDrift after moving to position5 -Setting PFS offset to :122.9 -readDrift script. Reference z position is:1867.15 -readDrift script. Cumulative drift is:-6.05 -Channel configuration set to: Brightfield: 29-Feb-2020 01:18:44 -Channel:Brightfield set at:29-Feb-2020 01:18:44 -Exposure time:30ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: GFPFast: 29-Feb-2020 01:18:49 -Channel:GFPFast set at:29-Feb-2020 01:18:49 -Exposure time:30ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: mCherry: 29-Feb-2020 01:18:54 -Channel:mCherry set at:29-Feb-2020 01:18:55 -Exposure time:100ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Position:6,pos006 -Position group:2 -Channel configuration set to: Brightfield: 29-Feb-2020 01:19:00 -Moved to X:-187, Y:-470 -Call to readDrift after moving to position6 -Setting PFS offset to :119.6 -readDrift script. Reference z position is:1864.05 -readDrift script. Cumulative drift is:-4.725 -Channel configuration set to: Brightfield: 29-Feb-2020 01:19:05 -Channel:Brightfield set at:29-Feb-2020 01:19:05 -Exposure time:30ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: GFPFast: 29-Feb-2020 01:19:10 -Channel:GFPFast set at:29-Feb-2020 01:19:10 -Exposure time:30ms -Exposure time:30ms -captureStack (Batman). Z drive position before stack capture is: 1859.075. 29-Feb-2020 01:19:13 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:19:13 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1859.05. 29-Feb-2020 01:19:13 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:19:13 -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: mCherry: 29-Feb-2020 01:19:18 -Channel:mCherry set at:29-Feb-2020 01:19:18 -Exposure time:100ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Position:7,pos007 -Position group:3 -Channel configuration set to: Brightfield: 29-Feb-2020 01:19:25 -Moved to X:-731, Y:916 -Call to readDrift after moving to position7 -Setting PFS offset to :117.05 -readDrift script. Reference z position is:1867.05 -readDrift script. Cumulative drift is:-7.625 -Channel configuration set to: Brightfield: 29-Feb-2020 01:19:33 -Channel:Brightfield set at:29-Feb-2020 01:19:33 -Exposure time:30ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: GFPFast: 29-Feb-2020 01:19:38 -Channel:GFPFast set at:29-Feb-2020 01:19:39 -Exposure time:30ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: mCherry: 29-Feb-2020 01:19:44 -Channel:mCherry set at:29-Feb-2020 01:19:44 -Exposure time:100ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Position:8,pos008 -Position group:3 -Channel configuration set to: Brightfield: 29-Feb-2020 01:19:49 -Moved to X:-1003, Y:1178 -Call to readDrift after moving to position8 -Setting PFS offset to :121.7 -readDrift script. Reference z position is:1866.425 -readDrift script. Cumulative drift is:-4.275 -Channel configuration set to: Brightfield: 29-Feb-2020 01:19:54 -Channel:Brightfield set at:29-Feb-2020 01:19:54 -Exposure time:30ms -Exposure time:30ms -captureStack (Batman). Z drive position before stack capture is: 1861.825. 29-Feb-2020 01:19:58 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:19:58 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1861.85. 29-Feb-2020 01:19:58 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:19:58 -Channel configuration set to: GFPFast: 29-Feb-2020 01:20:03 -Channel:GFPFast set at:29-Feb-2020 01:20:03 -Exposure time:30ms -Exposure time:30ms -captureStack (Batman). Z drive position before stack capture is: 1861.8. 29-Feb-2020 01:20:06 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:20:06 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1861.825. 29-Feb-2020 01:20:07 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:20:07 -Channel configuration set to: mCherry: 29-Feb-2020 01:20:08 -Channel:mCherry set at:29-Feb-2020 01:20:09 -Exposure time:100ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Position:9,pos009 -Position group:3 -Channel configuration set to: Brightfield: 29-Feb-2020 01:20:14 -Moved to X:-568, Y:1157 -Call to readDrift after moving to position9 -readDrift script. Reference z position is:1868.45 -readDrift script. Cumulative drift is:-5.525 -Channel configuration set to: Brightfield: 29-Feb-2020 01:20:22 -Channel:Brightfield set at:29-Feb-2020 01:20:23 -Exposure time:30ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: GFPFast: 29-Feb-2020 01:20:28 -Channel:GFPFast set at:29-Feb-2020 01:20:28 -Exposure time:30ms -Exposure time:30ms -captureStack (Batman). Z drive position before stack capture is: 1862.95. 29-Feb-2020 01:20:31 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:20:31 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1862.95. 29-Feb-2020 01:20:31 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:20:31 -Channel configuration set to: mCherry: 29-Feb-2020 01:20:32 -Channel:mCherry set at:29-Feb-2020 01:20:33 -Exposure time:100ms -Exposure time:100ms -captureStack (Batman). Z drive position before stack capture is: 1863. 29-Feb-2020 01:20:33 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:20:33 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1863. 29-Feb-2020 01:20:33 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:20:33 -Position group_1. Channel:Brightfield -Maximum intensity measured in position group_1_is:0 -Channel does not use Smart EM camera mode. Settings remain unchanged. -Position group_1. Channel:GFPFast -Maximum intensity measured in position group_1_is:0 -Channel does not use Smart EM camera mode. Settings remain unchanged. -Position group_1. Channel:mCherry -Maximum intensity measured in position group_1_is:0 -Channel does not use Smart EM camera mode. Settings remain unchanged. -Position group_1. Channel:Brightfield -Maximum intensity measured in position group_1_is:0 -Channel does not use Smart EM camera mode. Settings remain unchanged. -Position group_1. Channel:GFPFast -Maximum intensity measured in position group_1_is:0 -Channel does not use Smart EM camera mode. Settings remain unchanged. -Position group_1. Channel:mCherry -Maximum intensity measured in position group_1_is:0 -Channel does not use Smart EM camera mode. Settings remain unchanged. -Position group_1. Channel:Brightfield -Maximum intensity measured in position group_1_is:0 -Channel does not use Smart EM camera mode. Settings remain unchanged. -Position group_1. Channel:GFPFast -Maximum intensity measured in position group_1_is:0 -Channel does not use Smart EM camera mode. Settings remain unchanged. -Position group_1. Channel:mCherry -Maximum intensity measured in position group_1_is:0 -Channel does not use Smart EM camera mode. Settings remain unchanged. -Timepoint:1 completed at:29-Feb-2020 01:20:35 -Time since start of timelapse:224.2978 -PFS is locked -Time to next time point:-104.2112 - -------Time point_2------ -Memory available for all arrays: 13728235520 -Memory used by Matlab: 2301521920 -Position:1,pos001 -Position group:1 -Channel configuration set to: Brightfield: 29-Feb-2020 01:20:36 -Moved to X:568, Y:1302 -Call to readDrift after moving to position1 -Setting PFS offset to :122.45 -readDrift script. Reference z position is:1876.5 -readDrift script. Cumulative drift is:-6 -Channel configuration set to: Brightfield: 29-Feb-2020 01:20:40 -Channel:Brightfield set at:29-Feb-2020 01:20:40 -Exposure time:30ms -Exposure time:30ms -captureStack (Batman). Z drive position before stack capture is: 1870.6. 29-Feb-2020 01:20:41 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:20:41 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1870.575. 29-Feb-2020 01:20:41 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:20:41 -Channel configuration set to: GFPFast: 29-Feb-2020 01:20:42 -Channel:GFPFast set at:29-Feb-2020 01:20:42 -Exposure time:30ms -Exposure time:30ms -captureStack (Batman). Z drive position before stack capture is: 1870.575. 29-Feb-2020 01:20:43 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:20:43 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1870.575. 29-Feb-2020 01:20:43 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:20:43 -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: mCherry: 29-Feb-2020 01:20:48 -Channel:mCherry set at:29-Feb-2020 01:20:48 -Exposure time:100ms -Exposure time:100ms -captureStack (Batman). Z drive position before stack capture is: 1870.575. 29-Feb-2020 01:20:52 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:20:52 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1870.575. 29-Feb-2020 01:20:52 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:20:52 -Position:2,pos002 -Position group:1 -Channel configuration set to: Brightfield: 29-Feb-2020 01:20:56 -Moved to X:1267, Y:1302 -Call to readDrift after moving to position2 -Setting PFS offset to :119.95 -readDrift script. Reference z position is:1880.125 -readDrift script. Cumulative drift is:-9.375 -Channel configuration set to: Brightfield: 29-Feb-2020 01:21:04 -Channel:Brightfield set at:29-Feb-2020 01:21:04 -Exposure time:30ms -Exposure time:30ms -captureStack (Batman). Z drive position before stack capture is: 1870.9. 29-Feb-2020 01:21:10 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:21:10 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1870.9. 29-Feb-2020 01:21:10 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:21:10 -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: GFPFast: 29-Feb-2020 01:21:15 -Channel:GFPFast set at:29-Feb-2020 01:21:15 -Exposure time:30ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: mCherry: 29-Feb-2020 01:21:20 -Channel:mCherry set at:29-Feb-2020 01:21:21 -Exposure time:100ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Position:3,pos003 -Position group:1 -Channel configuration set to: Brightfield: 29-Feb-2020 01:21:27 -Moved to X:1026, Y:977 -Call to readDrift after moving to position3 -Setting PFS offset to :120.1 -readDrift script. Reference z position is:1877.575 -readDrift script. Cumulative drift is:-2.825 -Channel configuration set to: Brightfield: 29-Feb-2020 01:21:35 -Channel:Brightfield set at:29-Feb-2020 01:21:35 -Exposure time:30ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: GFPFast: 29-Feb-2020 01:21:40 -Channel:GFPFast set at:29-Feb-2020 01:21:41 -Exposure time:30ms -Exposure time:30ms -captureStack (Batman). Z drive position before stack capture is: 1874.825. 29-Feb-2020 01:21:41 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:21:41 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1874.75. 29-Feb-2020 01:21:42 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:21:42 -Channel configuration set to: mCherry: 29-Feb-2020 01:21:43 -Channel:mCherry set at:29-Feb-2020 01:21:43 -Exposure time:100ms -Exposure time:100ms -captureStack (Batman). Z drive position before stack capture is: 1874.775. 29-Feb-2020 01:21:44 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:21:44 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1874.75. 29-Feb-2020 01:21:44 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:21:44 -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Position:4,pos004 -Position group:2 -Channel configuration set to: Brightfield: 29-Feb-2020 01:21:50 -Moved to X:540, Y:-347 -Call to readDrift after moving to position4 -Setting PFS offset to :121.2 -readDrift script. Reference z position is:1868.725 -readDrift script. Cumulative drift is:3.175 -Channel configuration set to: Brightfield: 29-Feb-2020 01:21:55 -Channel:Brightfield set at:29-Feb-2020 01:21:55 -Exposure time:30ms -Exposure time:30ms -captureStack (Batman). Z drive position before stack capture is: 1863.425. 29-Feb-2020 01:21:55 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:21:55 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1863.45. 29-Feb-2020 01:21:56 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:21:56 -Channel configuration set to: GFPFast: 29-Feb-2020 01:21:56 -Channel:GFPFast set at:29-Feb-2020 01:21:57 -Exposure time:30ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: mCherry: 29-Feb-2020 01:22:02 -Channel:mCherry set at:29-Feb-2020 01:22:02 -Exposure time:100ms -Exposure time:100ms -captureStack (Batman). Z drive position before stack capture is: 1863.4. 29-Feb-2020 01:22:04 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:22:04 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1863.4. 29-Feb-2020 01:22:04 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:22:04 -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Position:5,pos005 -Position group:2 -Channel configuration set to: Brightfield: 29-Feb-2020 01:22:11 -Moved to X:510, Y:-687 -Call to readDrift after moving to position5 -Setting PFS offset to :122.9 -readDrift script. Reference z position is:1867.15 -readDrift script. Cumulative drift is:-4.2 -Channel configuration set to: Brightfield: 29-Feb-2020 01:22:19 -Channel:Brightfield set at:29-Feb-2020 01:22:19 -Exposure time:30ms -Exposure time:30ms -captureStack (Batman). Z drive position before stack capture is: 1863.275. 29-Feb-2020 01:22:22 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:22:22 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1863.275. 29-Feb-2020 01:22:23 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:22:23 -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: GFPFast: 29-Feb-2020 01:22:28 -Channel:GFPFast set at:29-Feb-2020 01:22:28 -Exposure time:30ms -Exposure time:30ms -captureStack (Batman). Z drive position before stack capture is: 1863.275. 29-Feb-2020 01:22:31 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:22:31 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1863.275. 29-Feb-2020 01:22:32 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:22:32 -Channel configuration set to: mCherry: 29-Feb-2020 01:22:34 -Channel:mCherry set at:29-Feb-2020 01:22:35 -Exposure time:100ms -Exposure time:100ms -captureStack (Batman). Z drive position before stack capture is: 1863.275. 29-Feb-2020 01:22:35 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:22:35 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1863.275. 29-Feb-2020 01:22:35 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:22:35 -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Position:6,pos006 -Position group:2 -Channel configuration set to: Brightfield: 29-Feb-2020 01:22:42 -Moved to X:-187, Y:-470 -Call to readDrift after moving to position6 -Setting PFS offset to :119.6 -readDrift script. Reference z position is:1864.05 -readDrift script. Cumulative drift is:-2 -Channel configuration set to: Brightfield: 29-Feb-2020 01:22:50 -Channel:Brightfield set at:29-Feb-2020 01:22:51 -Exposure time:30ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: GFPFast: 29-Feb-2020 01:22:56 -Channel:GFPFast set at:29-Feb-2020 01:22:56 -Exposure time:30ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: mCherry: 29-Feb-2020 01:23:01 -Channel:mCherry set at:29-Feb-2020 01:23:01 -Exposure time:100ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Position:7,pos007 -Position group:3 -Channel configuration set to: Brightfield: 29-Feb-2020 01:23:07 -Moved to X:-731, Y:916 -Call to readDrift after moving to position7 -Setting PFS offset to :117.05 -readDrift script. Reference z position is:1867.05 -readDrift script. Cumulative drift is:-6.275 -Channel configuration set to: Brightfield: 29-Feb-2020 01:23:16 -Channel:Brightfield set at:29-Feb-2020 01:23:16 -Exposure time:30ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: GFPFast: 29-Feb-2020 01:23:21 -Channel:GFPFast set at:29-Feb-2020 01:23:21 -Exposure time:30ms -Exposure time:30ms -captureStack (Batman). Z drive position before stack capture is: 1860.775. 29-Feb-2020 01:23:22 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:23:22 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1860.775. 29-Feb-2020 01:23:23 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:23:23 -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: mCherry: 29-Feb-2020 01:23:28 -Channel:mCherry set at:29-Feb-2020 01:23:28 -Exposure time:100ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Position:8,pos008 -Position group:3 -Channel configuration set to: Brightfield: 29-Feb-2020 01:23:34 -Moved to X:-1003, Y:1178 -Call to readDrift after moving to position8 -Setting PFS offset to :121.7 -readDrift script. Reference z position is:1866.425 -readDrift script. Cumulative drift is:-2.825 -PFS not locked. Waiting for lock... -PFS took 0.600000 seconds to lock. -Channel configuration set to: Brightfield: 29-Feb-2020 01:23:43 -Channel:Brightfield set at:29-Feb-2020 01:23:43 -Exposure time:30ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: GFPFast: 29-Feb-2020 01:23:48 -Channel:GFPFast set at:29-Feb-2020 01:23:49 -Exposure time:30ms -Exposure time:30ms -captureStack (Batman). Z drive position before stack capture is: 1862.925. 29-Feb-2020 01:23:54 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:23:54 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1862.925. 29-Feb-2020 01:23:54 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:23:54 -Channel configuration set to: mCherry: 29-Feb-2020 01:23:55 -Channel:mCherry set at:29-Feb-2020 01:23:55 -Exposure time:100ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Position:9,pos009 -Position group:3 -Channel configuration set to: Brightfield: 29-Feb-2020 01:24:01 -Moved to X:-568, Y:1157 -Call to readDrift after moving to position9 -readDrift script. Reference z position is:1868.45 -readDrift script. Cumulative drift is:-6.2 -Channel configuration set to: Brightfield: 29-Feb-2020 01:24:10 -Channel:Brightfield set at:29-Feb-2020 01:24:10 -Exposure time:30ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Channel configuration set to: GFPFast: 29-Feb-2020 01:24:15 -Channel:GFPFast set at:29-Feb-2020 01:24:15 -Exposure time:30ms -Exposure time:30ms -captureStack (Batman). Z drive position before stack capture is: 1862.2. 29-Feb-2020 01:24:18 -captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:24:18 -captureStackMM2 (Batmanl). Z drive position before stack capture is: 1862.2. 29-Feb-2020 01:24:18 -captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:24:18 -Channel configuration set to: mCherry: 29-Feb-2020 01:24:19 -Channel:mCherry set at:29-Feb-2020 01:24:19 -Exposure time:100ms -WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: -java.lang.Exception: Wait for device "XYStage" timed out after 5000ms - at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) -Position group_1. Channel:Brightfield -Maximum intensity measured in position group_1_is:0 -Channel does not use Smart EM camera mode. Settings remain unchanged. -Position group_1. Channel:GFPFast -Maximum intensity measured in position group_1_is:0 -Channel does not use Smart EM camera mode. Settings remain unchanged. -Position group_1. Channel:mCherry -Maximum intensity measured in position group_1_is:0 -Channel does not use Smart EM camera mode. Settings remain unchanged. -Position group_1. Channel:Brightfield -Maximum intensity measured in position group_1_is:0 -Channel does not use Smart EM camera mode. Settings remain unchanged. -Position group_1. Channel:GFPFast -Maximum intensity measured in position group_1_is:0 -Channel does not use Smart EM camera mode. Settings remain unchanged. -Position group_1. Channel:mCherry -Maximum intensity measured in position group_1_is:0 -Channel does not use Smart EM camera mode. Settings remain unchanged. -Position group_1. Channel:Brightfield -Maximum intensity measured in position group_1_is:0 -Channel does not use Smart EM camera mode. Settings remain unchanged. -Position group_1. Channel:GFPFast -Maximum intensity measured in position group_1_is:0 -Channel does not use Smart EM camera mode. Settings remain unchanged. -Position group_1. Channel:mCherry -Maximum intensity measured in position group_1_is:0 -Channel does not use Smart EM camera mode. Settings remain unchanged. -Timepoint:2 completed at:29-Feb-2020 01:24:25 -Time since start of timelapse:454.3878 -PFS is locked -Time to next time point:-110.0053 -Experiment completed diff --git a/examples/parsers/swainlab_logfile_header_example.log b/examples/parsers/swainlab_logfile_header_example.log deleted file mode 100644 index 8a7197bf9c0f4811afe252499ff5ba2deb53354a..0000000000000000000000000000000000000000 --- a/examples/parsers/swainlab_logfile_header_example.log +++ /dev/null @@ -1,123 +0,0 @@ -2022-10-10 15:31:27,350 - INFO -Swain Lab microscope experiment log file -GIT commit: e5d5e33 fix: changes to a few issues with focus control on Batman. -Microscope name: Batman -Date: 022-10-10 15:31:27 -Log file path: D:\AcquisitionDataBatman\Swain Lab\Ivan\RAW DATA\2022\Oct\10-Oct-2022\pH_med_to_low00\pH_med_to_low.log -Micromanager config file: C:\Users\Public\Microscope control\Micromanager config files\Batman_python_15_4_22.cfg -Omero project: Default project -Omero tags: -Experiment details: Effect on growth and cytoplasmic pH of switch from normal pH (4.25) media to higher pH (5.69). Switching is run using the Oxygen software ------Acquisition settings----- - -2022-10-10 15:31:27,350 - INFO Image Configs: -Image config,Channel,Description,Exposure (ms), Number of Z sections,Z spacing (um),Sectioning method -brightfield1,Brightfield,Default bright field config,30,5,0.6,PIFOC -pHluorin405_0_4,pHluorin405,Phluorin excitation from 405 LED 0.4v and 10ms exposure,5,1,0.6,PIFOC -pHluorin488_0_4,GFPFast,Phluorin excitation from 488 LED 0.4v,10,1,0.6,PIFOC -cy5,cy5,Default cy5,30,1,0.6,PIFOC - -Device properties: -Image config,device,property,value -pHluorin405_0_4,DTOL-DAC-1,Volts,0.4 -pHluorin488_0_4,DTOL-DAC-2,Volts,0.4 -cy5,DTOL-DAC-3,Volts,4 - -2022-10-10 15:31:27,353 - INFO -group: YST_247 field: position -Name, X, Y, Z, Autofocus offset -YST_247_001,-8968,-3319,2731.125040696934,123.25 -YST_247_002,-8953,-3091,2731.3000406995416,123.25 -YST_247_003,-8954,-2849,2731.600040704012,122.8 -YST_247_004,-8941,-2611,2730.7750406917185,122.8 -YST_247_005,-8697,-2541,2731.4500407017767,118.6 -group: YST_247 field: time -start: 0 -interval: 300 -frames: 180 - -group: YST_247 field: config -brightfield1: 0xfffffffffffffffffffffffffffffffffffffffffffff -pHluorin405_0_4: 0xfffffffffffffffffffffffffffffffffffffffffffff -pHluorin488_0_4: 0xfffffffffffffffffffffffffffffffffffffffffffff -cy5: 0xfffffffffffffffffffffffffffffffffffffffffffff - -2022-10-10 15:31:27,356 - INFO -group: YST_1510 field: position -Name,X,Y,Z,Autofocus offset -YST_1510_001,-6450,-230,2343.300034917891,112.55 -YST_1510_002,-6450,-436,2343.350034918636,112.55 -YST_1510_003,-6450,-639,2344.000034928322,116.8 -YST_1510_004,-6450,-831,2344.250034932047,116.8 -YST_1510_005,-6848,-536,2343.3250349182636,110 -group: YST_1510 field: time -start: 0 -interval: 300 -frames: 180 - -group: YST_1510 field: config -brightfield1: 0xfffffffffffffffffffffffffffffffffffffffffffff -pHluorin405_0_4: 0xfffffffffffffffffffffffffffffffffffffffffffff -pHluorin488_0_4: 0xfffffffffffffffffffffffffffffffffffffffffffff -cy5: 0xfffffffffffffffffffffffffffffffffffffffffffff -2022-10-10 15:31:27,359 - INFO -group: YST_1511 field: position -Name, X, Y, Z, Autofocus offset -YST_1511_001,-10618,-1675,2716.900040484965,118.7 -YST_1511_002,-10618,-1914,2717.2250404898077,122.45 -YST_1511_003,-10367,-1695,2718.2500405050814,120.95 -YST_1511_004,-10367,-1937,2718.8250405136496,120.95 -YST_1511_005,-10092,-1757,2719.975040530786,119.45 - -group: YST_1511 field: time -start: 0 -interval: 300 -frames: 180 - -group: YST_1511 field: config -brightfield1: 0xfffffffffffffffffffffffffffffffffffffffffffff -pHluorin405_0_4: 0xfffffffffffffffffffffffffffffffffffffffffffff -pHluorin488_0_4: 0xfffffffffffffffffffffffffffffffffffffffffffff -cy5: 0xfffffffffffffffffffffffffffffffffffffffffffff - -2022-10-10 15:31:27,362 - INFO -group: YST_1512 field: position -Name,X,Y,Z,Autofocus offset -YST_1512_001,-8173,-2510,2339.0750348549336,115.65 -YST_1512_002,-8173,-2718,2338.0250348392874,110.8 -YST_1512_003,-8173,-2963,2336.625034818426,110.8 -YST_1512_004,-8457,-2963,2336.350034814328,110.9 -YST_1512_005,-8481,-2706,2337.575034832582,113.3 -group: YST_1512 field: time -start: 0 -interval: 300 -frames: 180 - -group: YST_1512 field: config -brightfield1: 0xfffffffffffffffffffffffffffffffffffffffffffff -pHluorin405_0_4: 0xfffffffffffffffffffffffffffffffffffffffffffff -pHluorin488_0_4: 0xfffffffffffffffffffffffffffffffffffffffffffff -cy5: 0xfffffffffffffffffffffffffffffffffffffffffffff - -2022-10-10 15:31:27,365 - INFO -group: YST_1513 field: position -Name,X,Y,Z,Autofocus offset -YST_1513_001,-6978,-2596,2339.8750348668545,113.3 -YST_1513_002,-6978,-2380,2340.500034876168,113.3 -YST_1513_003,-6971,-2163,2340.8750348817557,113.3 -YST_1513_004,-6971,-1892,2341.2500348873436,113.3 -YST_1513_005,-6692,-1892,2341.550034891814,113.3 -group: YST_1513 field: time -start: 0 -interval: 300 -frames: 180 - -group: YST_1513 field: config -brightfield1: 0xfffffffffffffffffffffffffffffffffffffffffffff -pHluorin405_0_4: 0xfffffffffffffffffffffffffffffffffffffffffffff -pHluorin488_0_4: 0xfffffffffffffffffffffffffffffffffffffffffffff -cy5: 0xfffffffffffffffffffffffffffffffffffffffffffff - -2022-10-10 15:31:27,365 - INFO -2022-10-10 15:31:27,365 - INFO ------Experiment started----- diff --git a/examples/run_jura.py b/examples/run_jura.py new file mode 100644 index 0000000000000000000000000000000000000000..93a08a95292927d2c77aa5e064edb9f58df276e0 --- /dev/null +++ b/examples/run_jura.py @@ -0,0 +1,16 @@ +from aliby.pipeline import PipelineParameters, Pipeline + +params = PipelineParameters.default( + general={ + "expt_id": 2172, + "distributed": 0, + "directory": ".", + "host": "staffa.bio.ed.ac.uk", + "username": pass, + "password": pass, + } +) +# specify OMERO_channels if the channels on OMERO have a different order from the logfiles +p = Pipeline(params) + +p.run() diff --git a/examples/run_local.py b/examples/run_local.py new file mode 100644 index 0000000000000000000000000000000000000000..214e9311a89081bda291d1f3381b2d04011fd452 --- /dev/null +++ b/examples/run_local.py @@ -0,0 +1,27 @@ +from pathlib import Path + +from aliby.pipeline import Pipeline, PipelineParameters +from postprocessor.grouper import Grouper + +omid = "25681_2022_04_30_flavin_htb2_glucose_10mgpL_01_00" + +h5dir = "/Users/pswain/wip/aliby_output/" +omero_dir = "/Users/pswain/wip/aliby_input/" + +# setup and run pipeline +params = PipelineParameters.default( + general={ + "expt_id": omero_dir + omid, + "distributed": 2, + "directory": h5dir, + # specify position to segment + "filter": "fy4_007", + # specify final time point + # "tps": 4, + }, +) + + +# initialise and run pipeline +p = Pipeline(params, OMERO_channels=["Brightfield", "Flavin"]) +p.run() diff --git a/examples/tiler/pypipeline_unit_test_00_000001_Brightfield_003.tif b/examples/tiler/pypipeline_unit_test_00_000001_Brightfield_003.tif deleted file mode 100755 index 569f33a72ab18f65c79e28e38869a4f17cd30cca..0000000000000000000000000000000000000000 Binary files a/examples/tiler/pypipeline_unit_test_00_000001_Brightfield_003.tif and /dev/null differ diff --git a/examples/tiler/pypipeline_unit_test_00_000001_Brightfield_003_square.tif b/examples/tiler/pypipeline_unit_test_00_000001_Brightfield_003_square.tif deleted file mode 100755 index f813c15a9c8aef8a6e629ff17e460aef4acdb630..0000000000000000000000000000000000000000 Binary files a/examples/tiler/pypipeline_unit_test_00_000001_Brightfield_003_square.tif and /dev/null differ