From 38681df9522f910b45a7d0a0dad1d470a24837da Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Al=C3=A1n=20Mu=C3=B1oz?= <alan.munoz@ed.ac.uk> Date: Thu, 22 Sep 2022 13:40:56 +0100 Subject: [PATCH] tweak(logfile_parser): migrate examples --- examples/cExperiment_log_001.txt | 276 ++++++++++++ examples/example_multiDGUI_acq.txt | 46 ++ examples/example_multiDGUI_log.txt | 651 +++++++++++++++++++++++++++++ 3 files changed, 973 insertions(+) create mode 100644 examples/cExperiment_log_001.txt create mode 100644 examples/example_multiDGUI_acq.txt create mode 100644 examples/example_multiDGUI_log.txt diff --git a/examples/cExperiment_log_001.txt b/examples/cExperiment_log_001.txt new file mode 100644 index 00000000..8db3a2e4 --- /dev/null +++ b/examples/cExperiment_log_001.txt @@ -0,0 +1,276 @@ + + +===================== +06-Jan-2020 18:30:59 Start creating new experiment using parameters: +Omero experiment name: 001 +Temporary working directory: C:06-Jan-2020 18:30:59 Processing position 2 (1108_002) +06-Jan-2020 18:31:00 Processing position 3 (1108_003) +06-Jan-2020 18:31:01 Processing position 4 (1109_004) +06-Jan-2020 18:31:02 Processing position 5 (1109_005) +06-Jan-2020 18:31:04 Processing position 6 (1109_006) +06-Jan-2020 18:31:05 Processing position 7 (1110_007) +06-Jan-2020 18:31:06 Processing position 8 (1110_008) +06-Jan-2020 18:31:07 Processing position 9 (1110_009) +06-Jan-2020 18:31:10 Successfully completed creating new experiment in 11 secs. +--------------------- + + +===================== +06-Jan-2020 18:31:33 Start selecting traps... +06-Jan-2020 18:31:33 Processing position 1 (1108_001) +06-Jan-2020 18:31:40 Remove trap at 550 1188 +06-Jan-2020 18:31:40 Remove trap at 733 1179 +06-Jan-2020 18:31:41 Remove trap at 384 1189 +06-Jan-2020 18:31:42 Remove trap at 201 1186 +06-Jan-2020 18:31:47 Processing position 2 (1108_002) +06-Jan-2020 18:31:52 Remove trap at 384 1060 +06-Jan-2020 18:31:54 Remove trap at 1081 571 +06-Jan-2020 18:32:01 Processing position 3 (1108_003) +06-Jan-2020 18:32:05 Remove trap at 948 1140 +06-Jan-2020 18:32:06 Remove trap at 1141 1174 +06-Jan-2020 18:32:17 Remove trap at 139 1111 +06-Jan-2020 18:32:18 Add trap at 130 1138 +06-Jan-2020 18:32:26 Processing position 4 (1109_004) +06-Jan-2020 18:32:32 Remove trap at 1176 388 +06-Jan-2020 18:32:39 Processing position 5 (1109_005) +06-Jan-2020 18:32:44 Remove trap at 1141 1135 +06-Jan-2020 18:32:51 Remove trap at 955 379 +06-Jan-2020 18:32:55 Processing position 6 (1109_006) +06-Jan-2020 18:33:00 Remove trap at 676 1177 +06-Jan-2020 18:33:01 Remove trap at 1111 1147 +06-Jan-2020 18:33:14 Processing position 7 (1110_007) +06-Jan-2020 18:33:20 Remove trap at 46 46 +06-Jan-2020 18:33:28 Remove trap at 1150 84 +06-Jan-2020 18:33:34 Processing position 8 (1110_008) +06-Jan-2020 18:33:49 Processing position 9 (1110_009) +06-Jan-2020 18:33:55 Add trap at 1153 1129 +06-Jan-2020 18:33:57 Remove trap at 1135 1141 +06-Jan-2020 18:33:57 Remove trap at 1176 1095 +06-Jan-2020 18:34:15 Successfully completed selecting traps in 2.7 mins. +--------------------- + + +===================== +06-Jan-2020 18:34:28 Start setting extraction parameters using parameters: +extractionParameters: { + extractFunction: extractCellDataStandardParfor + functionParameters: { + type: max + channels: 2 3 + nuclearMarkerChannel: NaN + maxPixOverlap: 5 + maxAllowedOverlap: 25 + } +} +06-Jan-2020 18:34:28 Processing position 1 (1108_001) +06-Jan-2020 18:34:28 Processing position 2 (1108_002) +06-Jan-2020 18:34:29 Processing position 3 (1108_003) +06-Jan-2020 18:34:29 Processing position 4 (1109_004) +06-Jan-2020 18:34:30 Processing position 5 (1109_005) +06-Jan-2020 18:34:30 Processing position 6 (1109_006) +06-Jan-2020 18:34:30 Processing position 7 (1110_007) +06-Jan-2020 18:34:31 Processing position 8 (1110_008) +06-Jan-2020 18:34:31 Processing position 9 (1110_009) +06-Jan-2020 18:34:33 Successfully completed setting extraction parameters in 5 secs. +--------------------- + + +===================== +07-Jan-2020 13:17:43 Start tracking traps in time... +07-Jan-2020 13:17:43 Processing position 1 (1108_001) +07-Jan-2020 13:23:31 Processing position 2 (1108_002) +07-Jan-2020 13:29:21 Processing position 3 (1108_003) +07-Jan-2020 13:35:13 Processing position 4 (1109_004) +07-Jan-2020 13:41:19 Processing position 5 (1109_005) +07-Jan-2020 13:47:09 Processing position 6 (1109_006) +07-Jan-2020 13:52:57 Processing position 7 (1110_007) +07-Jan-2020 13:58:41 Processing position 8 (1110_008) +07-Jan-2020 14:04:41 Processing position 9 (1110_009) +07-Jan-2020 14:10:38 Successfully completed tracking traps in time in 52.9 mins. +--------------------- + + +===================== +07-Jan-2020 14:10:38 Start baby segmentation... +07-Jan-2020 14:10:39 Processing position 1 (1108_001) +07-Jan-2020 14:14:32 cTimelapse: 210.344 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 14:18:30 cTimelapse: 240.345 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 14:22:31 cTimelapse: 272.459 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 14:26:32 cTimelapse: 303.876 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 14:30:34 cTimelapse: 336.470 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 14:32:32 Processing position 2 (1108_002) +07-Jan-2020 14:36:22 cTimelapse: 206.699 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 14:40:12 cTimelapse: 235.726 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 14:44:13 cTimelapse: 268.814 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 14:48:27 cTimelapse: 306.046 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 14:52:43 cTimelapse: 343.681 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 14:54:44 Processing position 3 (1108_003) +07-Jan-2020 14:58:47 cTimelapse: 214.895 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 15:02:44 cTimelapse: 247.137 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 15:06:47 cTimelapse: 280.902 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 15:10:51 cTimelapse: 314.796 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 15:15:13 cTimelapse: 354.774 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 15:17:16 Processing position 4 (1109_004) +07-Jan-2020 15:21:06 cTimelapse: 222.663 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 15:25:09 cTimelapse: 253.596 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 15:29:16 cTimelapse: 286.597 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 15:33:46 cTimelapse: 325.040 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 15:38:35 cTimelapse: 369.190 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 15:40:50 Processing position 5 (1109_005) +07-Jan-2020 15:45:01 cTimelapse: 235.110 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 15:49:23 cTimelapse: 268.760 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 15:53:50 cTimelapse: 304.703 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 15:58:15 cTimelapse: 339.861 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 16:02:47 cTimelapse: 377.877 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 16:04:53 Processing position 6 (1109_006) +07-Jan-2020 16:08:32 cTimelapse: 205.246 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 16:12:09 cTimelapse: 231.500 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 16:15:49 cTimelapse: 259.276 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 16:19:45 cTimelapse: 291.813 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 16:24:03 cTimelapse: 331.193 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 16:26:11 Processing position 7 (1110_007) +07-Jan-2020 16:29:30 cTimelapse: 222.990 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 16:32:46 cTimelapse: 238.288 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 16:36:03 cTimelapse: 255.524 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 16:39:21 cTimelapse: 275.165 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 16:42:40 cTimelapse: 297.244 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 16:44:14 Processing position 8 (1110_008) +07-Jan-2020 16:47:32 cTimelapse: 215.583 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 16:50:51 cTimelapse: 235.959 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 16:54:09 cTimelapse: 256.409 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 16:57:25 cTimelapse: 275.563 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 17:00:43 cTimelapse: 296.390 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 17:02:13 Processing position 9 (1110_009) +07-Jan-2020 17:05:35 cTimelapse: 225.847 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 17:08:54 cTimelapse: 245.291 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 17:12:17 cTimelapse: 266.060 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 17:15:41 cTimelapse: 288.448 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 17:19:04 cTimelapse: 311.290 MB; posOverviewGUI: 741.580 MB + +07-Jan-2020 17:20:36 Successfully completed baby segmentation in 3.2 hours. +--------------------- + + +===================== +07-Jan-2020 17:20:37 Start tracking cells using parameters: +Tracking threshold: 10 +07-Jan-2020 17:20:39 Processing position 1 (1108_001) +07-Jan-2020 17:21:23 Processing position 2 (1108_002) +07-Jan-2020 17:22:06 Processing position 3 (1108_003) +07-Jan-2020 17:22:49 Processing position 4 (1109_004) +07-Jan-2020 17:23:33 Processing position 5 (1109_005) +07-Jan-2020 17:24:18 Processing position 6 (1109_006) +07-Jan-2020 17:24:58 Processing position 7 (1110_007) +07-Jan-2020 17:25:25 Processing position 8 (1110_008) +07-Jan-2020 17:25:55 Processing position 9 (1110_009) +07-Jan-2020 17:26:25 Successfully completed tracking cells in 5.8 mins. +--------------------- + + +===================== +07-Jan-2020 17:26:25 Start autoselecting cells using parameters: +Fraction of timelapse that cells are present for: 0.5 +Number of frames a cell must be present: 540 +Cell must appear by frame: 540 +Cell must still be present by frame: 1 +Maximum number of cells: Inf +07-Jan-2020 17:26:27 Processing position 1 (1108_001) +07-Jan-2020 17:26:42 Processing position 2 (1108_002) +07-Jan-2020 17:26:58 Processing position 3 (1108_003) +07-Jan-2020 17:27:14 Processing position 4 (1109_004) +07-Jan-2020 17:27:31 Processing position 5 (1109_005) +07-Jan-2020 17:27:48 Processing position 6 (1109_006) +07-Jan-2020 17:28:03 Processing position 7 (1110_007) +07-Jan-2020 17:28:13 Processing position 8 (1110_008) +07-Jan-2020 17:28:25 Processing position 9 (1110_009) +07-Jan-2020 17:28:36 Successfully completed autoselecting cells in 2.2 mins. +--------------------- + + +===================== +07-Jan-2020 17:28:37 Start extracting cell information... +07-Jan-2020 17:28:39 Processing position 1 (1108_001) +07-Jan-2020 17:58:38 Processing position 2 (1108_002) +07-Jan-2020 18:28:43 Processing position 3 (1108_003) +07-Jan-2020 18:58:45 Processing position 4 (1109_004) +07-Jan-2020 19:29:03 Processing position 5 (1109_005) +07-Jan-2020 19:59:31 Processing position 6 (1109_006) +07-Jan-2020 20:29:01 Processing position 7 (1110_007) +07-Jan-2020 20:56:05 Processing position 8 (1110_008) +07-Jan-2020 21:23:53 Processing position 9 (1110_009) +07-Jan-2020 21:51:15 Successfully completed extracting cell information in 4.4 hours. +--------------------- + + +===================== +07-Jan-2020 21:51:16 Start baby lineage extraction... +07-Jan-2020 21:51:18 Processing position 1 (1108_001) +07-Jan-2020 21:52:37 Processing position 2 (1108_002) +07-Jan-2020 21:53:57 Processing position 3 (1108_003) +07-Jan-2020 21:55:16 Processing position 4 (1109_004) +07-Jan-2020 21:56:36 Processing position 5 (1109_005) +07-Jan-2020 21:57:59 Processing position 6 (1109_006) +07-Jan-2020 21:59:08 Processing position 7 (1110_007) +07-Jan-2020 21:59:50 Processing position 8 (1110_008) +07-Jan-2020 22:00:41 Processing position 9 (1110_009) +07-Jan-2020 22:01:26 Successfully completed baby lineage extraction in 10.2 mins. +--------------------- + + +===================== +07-Jan-2020 22:01:26 Start compiling cell information... +07-Jan-2020 22:01:28 Processing position 1 (1108_001) +07-Jan-2020 22:01:30 Processing position 2 (1108_002) +07-Jan-2020 22:01:33 Processing position 3 (1108_003) +07-Jan-2020 22:01:35 Processing position 4 (1109_004) +07-Jan-2020 22:01:38 Processing position 5 (1109_005) +07-Jan-2020 22:01:40 Processing position 6 (1109_006) +07-Jan-2020 22:01:42 Processing position 7 (1110_007) +07-Jan-2020 22:01:44 Processing position 8 (1110_008) +07-Jan-2020 22:01:46 Processing position 9 (1110_009) +07-Jan-2020 22:02:20 Successfully completed compiling cell information in 54 secs. +--------------------- diff --git a/examples/example_multiDGUI_acq.txt b/examples/example_multiDGUI_acq.txt new file mode 100644 index 00000000..e2d1e9b4 --- /dev/null +++ b/examples/example_multiDGUI_acq.txt @@ -0,0 +1,46 @@ +Channels: +Channel name, Exposure time, Skip, Z sect., Start time, Camera mode, EM gain, Voltage + Brightfield, 30, 1, 1, 1, 2, 270, 1.000 + GFPFast, 30, 1, 1, 1, 2, 270, 3.500 + mCherry, 100, 1, 1, 1, 2, 270, 2.500 +Z_sectioning: +Sections,Spacing,PFSon?,AnyZ?,Drift,Method + 3, 0.80, 1, 1, 0, 2 +Time_settings: +1,120,660,79200 +Points: +Position name, X position, Y position, Z position, PFS offset, Group, Brightfield, GFPFast, mCherry + pos001, 568.00, 1302.00, 1876.500, 122.450, 1, 30, 30, 100 + pos002, 1267.00, 1302.00, 1880.125, 119.950, 1, 30, 30, 100 + pos003, 1026.00, 977.00, 1877.575, 120.100, 1, 30, 30, 100 + pos004, 540.00, -347.00, 1868.725, 121.200, 2, 30, 30, 100 + pos005, 510.00, -687.00, 1867.150, 122.900, 2, 30, 30, 100 + pos006, -187.00, -470.00, 1864.050, 119.600, 2, 30, 30, 100 + pos007, -731.00, 916.00, 1867.050, 117.050, 3, 30, 30, 100 + pos008, -1003.00, 1178.00, 1866.425, 121.700, 3, 30, 30, 100 + pos009, -568.00, 1157.00, 1868.450, 119.350, 3, 30, 30, 100 + +Flow_control: +Syringe pump details: 2 pumps. +Pump states at beginning of experiment: +Pump port, Diameter, Current rate, Direction, Running, Contents + COM7, 14.43, 0.00, INF, 1, 2% glucose in SC + COM8, 14.43, 4.00, INF, 1, 0.1% glucose in SC + +Dynamic flow details: +Number of pump changes: +1 +Switching parameters: +Infuse/withdraw volumes: +50 +Infuse/withdraw rates: +100 +Times: +0 +Switched to: +2 +Switched from: +1 +Flow post switch: +0 +4 diff --git a/examples/example_multiDGUI_log.txt b/examples/example_multiDGUI_log.txt new file mode 100644 index 00000000..e4a242ca --- /dev/null +++ b/examples/example_multiDGUI_log.txt @@ -0,0 +1,651 @@ +Swain Lab microscope experiment log file +Swain lab microscope control software - MultiDGUI commit number:05903fb3769ccf612e7801b46e2248644ce7ca28 +Warning! - there are uncommitted changes - software may be different from the above commit +29-Feb-2020 +Microscope name is: Batman +Acquisition settings are saved in: +C:\path\to\example_multiDGUI_log.txt +Experiment details: +Aim: Strain: Comments: +Microscope setup for used channels: +Brightfield: +White LED +->(Polariser + Prism + condenser)] +->Filter block:[Dual GFP/mCherry exciter (59022x),Dual dichroic (89021bs),No emission filter] +->Emission filter wheel:[No filter in emission wheel] +GFPFast: +470nm LED +->Combiner cube:[480/40 exciter, 515LP dichroic->(455LP dichroic)] +->Filter block:[Dual GFP/mCherry exciter (59022x),Dual dichroic (89021bs),No emission filter] +->Emission filter wheel:[520/40 emission filter] +mCherry: +White LED +->Combiner cube:[No exciter, No reflecting dichroic->(515LP and 455LP dichroics)] +->Filter block:[Dual GFP/mCherry exciter (59022x),Dual dichroic (89021bs),No emission filter] +->Emission filter wheel:[632/60 emission filter] +Micromanager config file:C:\path\to\config_file.cfg +Omero project: +SteadystateGlucose +Omero tags: +29-Feb-2020,Batman,3 chamber,GFP,mCherry,1106.Mig2-GFP Mig1-mCherry,900.Mig1-GFP Msn2-mCherry,898.Msn2-GFP Mig1-mCherry,0.1% glucose,2% glucose, +Experiment started at:29-Feb-2020 01:16:51 + +------Time point_1------ +Memory available for all arrays: 14141227008 +Memory used by Matlab: 2237132800 +Switching pumps at 29-Feb-2020 01:16:52. Fast infusion/withdrawal step: Rate=100. Volume=50 +Pump switch complete at:29-Feb-2020 01:16:52 +Dominant pump is now: COM8 pumping at 4ul/min +Dominant medium is now: 0.1% glucose in SC +Position:1,pos001 +Position group:1 +Channel configuration set to: Brightfield: 29-Feb-2020 01:16:52 +Moved to X:568, Y:1302 +Call to readDrift after moving to position1 +Setting PFS offset to :122.45 +readDrift script. Reference z position is:1876.5 +readDrift script. Cumulative drift is:-5.9 +Channel configuration set to: Brightfield: 29-Feb-2020 01:17:01 +Channel:Brightfield set at:29-Feb-2020 01:17:01 +Exposure time:30ms +Channel configuration set to: GFPFast: 29-Feb-2020 01:17:06 +Channel:GFPFast set at:29-Feb-2020 01:17:06 +Exposure time:30ms +Exposure time:30ms +captureStack (Batman). Z drive position before stack capture is: 1870.575. 29-Feb-2020 01:17:10 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:17:10 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1870.575. 29-Feb-2020 01:17:10 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:17:10 +Channel configuration set to: mCherry: 29-Feb-2020 01:17:12 +Channel:mCherry set at:29-Feb-2020 01:17:12 +Exposure time:100ms +Exposure time:100ms +captureStack (Batman). Z drive position before stack capture is: 1870.575. 29-Feb-2020 01:17:12 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:17:12 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1870.55. 29-Feb-2020 01:17:13 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:17:13 +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Position:2,pos002 +Position group:1 +Channel configuration set to: Brightfield: 29-Feb-2020 01:17:18 +Moved to X:1267, Y:1302 +Call to readDrift after moving to position2 +Setting PFS offset to :119.95 +readDrift script. Reference z position is:1880.125 +readDrift script. Cumulative drift is:-9.725 +Channel configuration set to: Brightfield: 29-Feb-2020 01:17:27 +Channel:Brightfield set at:29-Feb-2020 01:17:27 +Exposure time:30ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: GFPFast: 29-Feb-2020 01:17:32 +Channel:GFPFast set at:29-Feb-2020 01:17:32 +Exposure time:30ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: mCherry: 29-Feb-2020 01:17:37 +Channel:mCherry set at:29-Feb-2020 01:17:38 +Exposure time:100ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Position:3,pos003 +Position group:1 +Channel configuration set to: Brightfield: 29-Feb-2020 01:17:43 +Moved to X:1026, Y:977 +Call to readDrift after moving to position3 +Setting PFS offset to :120.1 +readDrift script. Reference z position is:1877.575 +readDrift script. Cumulative drift is:-5.925 +Channel configuration set to: Brightfield: 29-Feb-2020 01:17:52 +Channel:Brightfield set at:29-Feb-2020 01:17:52 +Exposure time:30ms +Exposure time:30ms +captureStack (Batman). Z drive position before stack capture is: 1870.925. 29-Feb-2020 01:17:57 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:17:57 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1870.9. 29-Feb-2020 01:17:58 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:17:58 +Channel configuration set to: GFPFast: 29-Feb-2020 01:18:00 +Channel:GFPFast set at:29-Feb-2020 01:18:00 +Exposure time:30ms +Exposure time:30ms +captureStack (Batman). Z drive position before stack capture is: 1870.925. 29-Feb-2020 01:18:01 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:18:01 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1870.925. 29-Feb-2020 01:18:01 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:18:01 +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: mCherry: 29-Feb-2020 01:18:06 +Channel:mCherry set at:29-Feb-2020 01:18:07 +Exposure time:100ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Position:4,pos004 +Position group:2 +Channel configuration set to: Brightfield: 29-Feb-2020 01:18:14 +Moved to X:540, Y:-347 +Call to readDrift after moving to position4 +Setting PFS offset to :121.2 +readDrift script. Reference z position is:1868.725 +readDrift script. Cumulative drift is:3.65 +PFS not locked. Waiting for lock... +PFS took 0.650000 seconds to lock. +Channel configuration set to: Brightfield: 29-Feb-2020 01:18:18 +Channel:Brightfield set at:29-Feb-2020 01:18:18 +Exposure time:30ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: GFPFast: 29-Feb-2020 01:18:23 +Channel:GFPFast set at:29-Feb-2020 01:18:23 +Exposure time:30ms +Exposure time:30ms +captureStack (Batman). Z drive position before stack capture is: 1862.575. 29-Feb-2020 01:18:28 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:18:28 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1862.55. 29-Feb-2020 01:18:28 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:18:28 +Channel configuration set to: mCherry: 29-Feb-2020 01:18:31 +Channel:mCherry set at:29-Feb-2020 01:18:31 +Exposure time:100ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Position:5,pos005 +Position group:2 +Channel configuration set to: Brightfield: 29-Feb-2020 01:18:37 +Moved to X:510, Y:-687 +Call to readDrift after moving to position5 +Setting PFS offset to :122.9 +readDrift script. Reference z position is:1867.15 +readDrift script. Cumulative drift is:-6.05 +Channel configuration set to: Brightfield: 29-Feb-2020 01:18:44 +Channel:Brightfield set at:29-Feb-2020 01:18:44 +Exposure time:30ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: GFPFast: 29-Feb-2020 01:18:49 +Channel:GFPFast set at:29-Feb-2020 01:18:49 +Exposure time:30ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: mCherry: 29-Feb-2020 01:18:54 +Channel:mCherry set at:29-Feb-2020 01:18:55 +Exposure time:100ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Position:6,pos006 +Position group:2 +Channel configuration set to: Brightfield: 29-Feb-2020 01:19:00 +Moved to X:-187, Y:-470 +Call to readDrift after moving to position6 +Setting PFS offset to :119.6 +readDrift script. Reference z position is:1864.05 +readDrift script. Cumulative drift is:-4.725 +Channel configuration set to: Brightfield: 29-Feb-2020 01:19:05 +Channel:Brightfield set at:29-Feb-2020 01:19:05 +Exposure time:30ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: GFPFast: 29-Feb-2020 01:19:10 +Channel:GFPFast set at:29-Feb-2020 01:19:10 +Exposure time:30ms +Exposure time:30ms +captureStack (Batman). Z drive position before stack capture is: 1859.075. 29-Feb-2020 01:19:13 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:19:13 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1859.05. 29-Feb-2020 01:19:13 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:19:13 +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: mCherry: 29-Feb-2020 01:19:18 +Channel:mCherry set at:29-Feb-2020 01:19:18 +Exposure time:100ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Position:7,pos007 +Position group:3 +Channel configuration set to: Brightfield: 29-Feb-2020 01:19:25 +Moved to X:-731, Y:916 +Call to readDrift after moving to position7 +Setting PFS offset to :117.05 +readDrift script. Reference z position is:1867.05 +readDrift script. Cumulative drift is:-7.625 +Channel configuration set to: Brightfield: 29-Feb-2020 01:19:33 +Channel:Brightfield set at:29-Feb-2020 01:19:33 +Exposure time:30ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: GFPFast: 29-Feb-2020 01:19:38 +Channel:GFPFast set at:29-Feb-2020 01:19:39 +Exposure time:30ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: mCherry: 29-Feb-2020 01:19:44 +Channel:mCherry set at:29-Feb-2020 01:19:44 +Exposure time:100ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Position:8,pos008 +Position group:3 +Channel configuration set to: Brightfield: 29-Feb-2020 01:19:49 +Moved to X:-1003, Y:1178 +Call to readDrift after moving to position8 +Setting PFS offset to :121.7 +readDrift script. Reference z position is:1866.425 +readDrift script. Cumulative drift is:-4.275 +Channel configuration set to: Brightfield: 29-Feb-2020 01:19:54 +Channel:Brightfield set at:29-Feb-2020 01:19:54 +Exposure time:30ms +Exposure time:30ms +captureStack (Batman). Z drive position before stack capture is: 1861.825. 29-Feb-2020 01:19:58 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:19:58 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1861.85. 29-Feb-2020 01:19:58 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:19:58 +Channel configuration set to: GFPFast: 29-Feb-2020 01:20:03 +Channel:GFPFast set at:29-Feb-2020 01:20:03 +Exposure time:30ms +Exposure time:30ms +captureStack (Batman). Z drive position before stack capture is: 1861.8. 29-Feb-2020 01:20:06 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:20:06 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1861.825. 29-Feb-2020 01:20:07 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:20:07 +Channel configuration set to: mCherry: 29-Feb-2020 01:20:08 +Channel:mCherry set at:29-Feb-2020 01:20:09 +Exposure time:100ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Position:9,pos009 +Position group:3 +Channel configuration set to: Brightfield: 29-Feb-2020 01:20:14 +Moved to X:-568, Y:1157 +Call to readDrift after moving to position9 +readDrift script. Reference z position is:1868.45 +readDrift script. Cumulative drift is:-5.525 +Channel configuration set to: Brightfield: 29-Feb-2020 01:20:22 +Channel:Brightfield set at:29-Feb-2020 01:20:23 +Exposure time:30ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: GFPFast: 29-Feb-2020 01:20:28 +Channel:GFPFast set at:29-Feb-2020 01:20:28 +Exposure time:30ms +Exposure time:30ms +captureStack (Batman). Z drive position before stack capture is: 1862.95. 29-Feb-2020 01:20:31 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:20:31 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1862.95. 29-Feb-2020 01:20:31 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:20:31 +Channel configuration set to: mCherry: 29-Feb-2020 01:20:32 +Channel:mCherry set at:29-Feb-2020 01:20:33 +Exposure time:100ms +Exposure time:100ms +captureStack (Batman). Z drive position before stack capture is: 1863. 29-Feb-2020 01:20:33 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:20:33 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1863. 29-Feb-2020 01:20:33 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:20:33 +Position group_1. Channel:Brightfield +Maximum intensity measured in position group_1_is:0 +Channel does not use Smart EM camera mode. Settings remain unchanged. +Position group_1. Channel:GFPFast +Maximum intensity measured in position group_1_is:0 +Channel does not use Smart EM camera mode. Settings remain unchanged. +Position group_1. Channel:mCherry +Maximum intensity measured in position group_1_is:0 +Channel does not use Smart EM camera mode. Settings remain unchanged. +Position group_1. Channel:Brightfield +Maximum intensity measured in position group_1_is:0 +Channel does not use Smart EM camera mode. Settings remain unchanged. +Position group_1. Channel:GFPFast +Maximum intensity measured in position group_1_is:0 +Channel does not use Smart EM camera mode. Settings remain unchanged. +Position group_1. Channel:mCherry +Maximum intensity measured in position group_1_is:0 +Channel does not use Smart EM camera mode. Settings remain unchanged. +Position group_1. Channel:Brightfield +Maximum intensity measured in position group_1_is:0 +Channel does not use Smart EM camera mode. Settings remain unchanged. +Position group_1. Channel:GFPFast +Maximum intensity measured in position group_1_is:0 +Channel does not use Smart EM camera mode. Settings remain unchanged. +Position group_1. Channel:mCherry +Maximum intensity measured in position group_1_is:0 +Channel does not use Smart EM camera mode. Settings remain unchanged. +Timepoint:1 completed at:29-Feb-2020 01:20:35 +Time since start of timelapse:224.2978 +PFS is locked +Time to next time point:-104.2112 + +------Time point_2------ +Memory available for all arrays: 13728235520 +Memory used by Matlab: 2301521920 +Position:1,pos001 +Position group:1 +Channel configuration set to: Brightfield: 29-Feb-2020 01:20:36 +Moved to X:568, Y:1302 +Call to readDrift after moving to position1 +Setting PFS offset to :122.45 +readDrift script. Reference z position is:1876.5 +readDrift script. Cumulative drift is:-6 +Channel configuration set to: Brightfield: 29-Feb-2020 01:20:40 +Channel:Brightfield set at:29-Feb-2020 01:20:40 +Exposure time:30ms +Exposure time:30ms +captureStack (Batman). Z drive position before stack capture is: 1870.6. 29-Feb-2020 01:20:41 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:20:41 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1870.575. 29-Feb-2020 01:20:41 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:20:41 +Channel configuration set to: GFPFast: 29-Feb-2020 01:20:42 +Channel:GFPFast set at:29-Feb-2020 01:20:42 +Exposure time:30ms +Exposure time:30ms +captureStack (Batman). Z drive position before stack capture is: 1870.575. 29-Feb-2020 01:20:43 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:20:43 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1870.575. 29-Feb-2020 01:20:43 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:20:43 +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: mCherry: 29-Feb-2020 01:20:48 +Channel:mCherry set at:29-Feb-2020 01:20:48 +Exposure time:100ms +Exposure time:100ms +captureStack (Batman). Z drive position before stack capture is: 1870.575. 29-Feb-2020 01:20:52 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:20:52 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1870.575. 29-Feb-2020 01:20:52 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:20:52 +Position:2,pos002 +Position group:1 +Channel configuration set to: Brightfield: 29-Feb-2020 01:20:56 +Moved to X:1267, Y:1302 +Call to readDrift after moving to position2 +Setting PFS offset to :119.95 +readDrift script. Reference z position is:1880.125 +readDrift script. Cumulative drift is:-9.375 +Channel configuration set to: Brightfield: 29-Feb-2020 01:21:04 +Channel:Brightfield set at:29-Feb-2020 01:21:04 +Exposure time:30ms +Exposure time:30ms +captureStack (Batman). Z drive position before stack capture is: 1870.9. 29-Feb-2020 01:21:10 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:21:10 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1870.9. 29-Feb-2020 01:21:10 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:21:10 +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: GFPFast: 29-Feb-2020 01:21:15 +Channel:GFPFast set at:29-Feb-2020 01:21:15 +Exposure time:30ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: mCherry: 29-Feb-2020 01:21:20 +Channel:mCherry set at:29-Feb-2020 01:21:21 +Exposure time:100ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Position:3,pos003 +Position group:1 +Channel configuration set to: Brightfield: 29-Feb-2020 01:21:27 +Moved to X:1026, Y:977 +Call to readDrift after moving to position3 +Setting PFS offset to :120.1 +readDrift script. Reference z position is:1877.575 +readDrift script. Cumulative drift is:-2.825 +Channel configuration set to: Brightfield: 29-Feb-2020 01:21:35 +Channel:Brightfield set at:29-Feb-2020 01:21:35 +Exposure time:30ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: GFPFast: 29-Feb-2020 01:21:40 +Channel:GFPFast set at:29-Feb-2020 01:21:41 +Exposure time:30ms +Exposure time:30ms +captureStack (Batman). Z drive position before stack capture is: 1874.825. 29-Feb-2020 01:21:41 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:21:41 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1874.75. 29-Feb-2020 01:21:42 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:21:42 +Channel configuration set to: mCherry: 29-Feb-2020 01:21:43 +Channel:mCherry set at:29-Feb-2020 01:21:43 +Exposure time:100ms +Exposure time:100ms +captureStack (Batman). Z drive position before stack capture is: 1874.775. 29-Feb-2020 01:21:44 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:21:44 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1874.75. 29-Feb-2020 01:21:44 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:21:44 +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Position:4,pos004 +Position group:2 +Channel configuration set to: Brightfield: 29-Feb-2020 01:21:50 +Moved to X:540, Y:-347 +Call to readDrift after moving to position4 +Setting PFS offset to :121.2 +readDrift script. Reference z position is:1868.725 +readDrift script. Cumulative drift is:3.175 +Channel configuration set to: Brightfield: 29-Feb-2020 01:21:55 +Channel:Brightfield set at:29-Feb-2020 01:21:55 +Exposure time:30ms +Exposure time:30ms +captureStack (Batman). Z drive position before stack capture is: 1863.425. 29-Feb-2020 01:21:55 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:21:55 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1863.45. 29-Feb-2020 01:21:56 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:21:56 +Channel configuration set to: GFPFast: 29-Feb-2020 01:21:56 +Channel:GFPFast set at:29-Feb-2020 01:21:57 +Exposure time:30ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: mCherry: 29-Feb-2020 01:22:02 +Channel:mCherry set at:29-Feb-2020 01:22:02 +Exposure time:100ms +Exposure time:100ms +captureStack (Batman). Z drive position before stack capture is: 1863.4. 29-Feb-2020 01:22:04 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:22:04 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1863.4. 29-Feb-2020 01:22:04 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:22:04 +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Position:5,pos005 +Position group:2 +Channel configuration set to: Brightfield: 29-Feb-2020 01:22:11 +Moved to X:510, Y:-687 +Call to readDrift after moving to position5 +Setting PFS offset to :122.9 +readDrift script. Reference z position is:1867.15 +readDrift script. Cumulative drift is:-4.2 +Channel configuration set to: Brightfield: 29-Feb-2020 01:22:19 +Channel:Brightfield set at:29-Feb-2020 01:22:19 +Exposure time:30ms +Exposure time:30ms +captureStack (Batman). Z drive position before stack capture is: 1863.275. 29-Feb-2020 01:22:22 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:22:22 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1863.275. 29-Feb-2020 01:22:23 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:22:23 +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: GFPFast: 29-Feb-2020 01:22:28 +Channel:GFPFast set at:29-Feb-2020 01:22:28 +Exposure time:30ms +Exposure time:30ms +captureStack (Batman). Z drive position before stack capture is: 1863.275. 29-Feb-2020 01:22:31 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:22:31 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1863.275. 29-Feb-2020 01:22:32 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:22:32 +Channel configuration set to: mCherry: 29-Feb-2020 01:22:34 +Channel:mCherry set at:29-Feb-2020 01:22:35 +Exposure time:100ms +Exposure time:100ms +captureStack (Batman). Z drive position before stack capture is: 1863.275. 29-Feb-2020 01:22:35 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:22:35 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1863.275. 29-Feb-2020 01:22:35 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:22:35 +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Position:6,pos006 +Position group:2 +Channel configuration set to: Brightfield: 29-Feb-2020 01:22:42 +Moved to X:-187, Y:-470 +Call to readDrift after moving to position6 +Setting PFS offset to :119.6 +readDrift script. Reference z position is:1864.05 +readDrift script. Cumulative drift is:-2 +Channel configuration set to: Brightfield: 29-Feb-2020 01:22:50 +Channel:Brightfield set at:29-Feb-2020 01:22:51 +Exposure time:30ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: GFPFast: 29-Feb-2020 01:22:56 +Channel:GFPFast set at:29-Feb-2020 01:22:56 +Exposure time:30ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: mCherry: 29-Feb-2020 01:23:01 +Channel:mCherry set at:29-Feb-2020 01:23:01 +Exposure time:100ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Position:7,pos007 +Position group:3 +Channel configuration set to: Brightfield: 29-Feb-2020 01:23:07 +Moved to X:-731, Y:916 +Call to readDrift after moving to position7 +Setting PFS offset to :117.05 +readDrift script. Reference z position is:1867.05 +readDrift script. Cumulative drift is:-6.275 +Channel configuration set to: Brightfield: 29-Feb-2020 01:23:16 +Channel:Brightfield set at:29-Feb-2020 01:23:16 +Exposure time:30ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: GFPFast: 29-Feb-2020 01:23:21 +Channel:GFPFast set at:29-Feb-2020 01:23:21 +Exposure time:30ms +Exposure time:30ms +captureStack (Batman). Z drive position before stack capture is: 1860.775. 29-Feb-2020 01:23:22 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:23:22 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1860.775. 29-Feb-2020 01:23:23 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:23:23 +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: mCherry: 29-Feb-2020 01:23:28 +Channel:mCherry set at:29-Feb-2020 01:23:28 +Exposure time:100ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Position:8,pos008 +Position group:3 +Channel configuration set to: Brightfield: 29-Feb-2020 01:23:34 +Moved to X:-1003, Y:1178 +Call to readDrift after moving to position8 +Setting PFS offset to :121.7 +readDrift script. Reference z position is:1866.425 +readDrift script. Cumulative drift is:-2.825 +PFS not locked. Waiting for lock... +PFS took 0.600000 seconds to lock. +Channel configuration set to: Brightfield: 29-Feb-2020 01:23:43 +Channel:Brightfield set at:29-Feb-2020 01:23:43 +Exposure time:30ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: GFPFast: 29-Feb-2020 01:23:48 +Channel:GFPFast set at:29-Feb-2020 01:23:49 +Exposure time:30ms +Exposure time:30ms +captureStack (Batman). Z drive position before stack capture is: 1862.925. 29-Feb-2020 01:23:54 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:23:54 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1862.925. 29-Feb-2020 01:23:54 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:23:54 +Channel configuration set to: mCherry: 29-Feb-2020 01:23:55 +Channel:mCherry set at:29-Feb-2020 01:23:55 +Exposure time:100ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Position:9,pos009 +Position group:3 +Channel configuration set to: Brightfield: 29-Feb-2020 01:24:01 +Moved to X:-568, Y:1157 +Call to readDrift after moving to position9 +readDrift script. Reference z position is:1868.45 +readDrift script. Cumulative drift is:-6.2 +Channel configuration set to: Brightfield: 29-Feb-2020 01:24:10 +Channel:Brightfield set at:29-Feb-2020 01:24:10 +Exposure time:30ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Channel configuration set to: GFPFast: 29-Feb-2020 01:24:15 +Channel:GFPFast set at:29-Feb-2020 01:24:15 +Exposure time:30ms +Exposure time:30ms +captureStack (Batman). Z drive position before stack capture is: 1862.2. 29-Feb-2020 01:24:18 +captureStack (Batman). Starting PIFOC position is: 0. 29-Feb-2020 01:24:18 +captureStackMM2 (Batmanl). Z drive position before stack capture is: 1862.2. 29-Feb-2020 01:24:18 +captureStack (Batgirl). Starting Z stage position is: 50. 29-Feb-2020 01:24:18 +Channel configuration set to: mCherry: 29-Feb-2020 01:24:19 +Channel:mCherry set at:29-Feb-2020 01:24:19 +Exposure time:100ms +WARNING - ERROR CAUGHT - NO IMAGE CAPTURED TO THIS CHANNEL AND TIMEPOINT. Error message:Java exception occurred: +java.lang.Exception: Wait for device "XYStage" timed out after 5000ms + at mmcorej.MMCoreJJNI.CMMCore_waitForSystem(Native Method) +Position group_1. Channel:Brightfield +Maximum intensity measured in position group_1_is:0 +Channel does not use Smart EM camera mode. Settings remain unchanged. +Position group_1. Channel:GFPFast +Maximum intensity measured in position group_1_is:0 +Channel does not use Smart EM camera mode. Settings remain unchanged. +Position group_1. Channel:mCherry +Maximum intensity measured in position group_1_is:0 +Channel does not use Smart EM camera mode. Settings remain unchanged. +Position group_1. Channel:Brightfield +Maximum intensity measured in position group_1_is:0 +Channel does not use Smart EM camera mode. Settings remain unchanged. +Position group_1. Channel:GFPFast +Maximum intensity measured in position group_1_is:0 +Channel does not use Smart EM camera mode. Settings remain unchanged. +Position group_1. Channel:mCherry +Maximum intensity measured in position group_1_is:0 +Channel does not use Smart EM camera mode. Settings remain unchanged. +Position group_1. Channel:Brightfield +Maximum intensity measured in position group_1_is:0 +Channel does not use Smart EM camera mode. Settings remain unchanged. +Position group_1. Channel:GFPFast +Maximum intensity measured in position group_1_is:0 +Channel does not use Smart EM camera mode. Settings remain unchanged. +Position group_1. Channel:mCherry +Maximum intensity measured in position group_1_is:0 +Channel does not use Smart EM camera mode. Settings remain unchanged. +Timepoint:2 completed at:29-Feb-2020 01:24:25 +Time since start of timelapse:454.3878 +PFS is locked +Time to next time point:-110.0053 +Experiment completed -- GitLab