diff --git a/aliby/io/image.py b/aliby/io/image.py new file mode 100644 index 0000000000000000000000000000000000000000..ded8d8b9d6222175efd43d8fd2e8b3cf5a78c96a --- /dev/null +++ b/aliby/io/image.py @@ -0,0 +1,107 @@ +#!/usr/bin/env python3 + +from aliby.experiment import get_data_lazy_local +from pathlib import Path +from tifffile import TiffFile +from pathlib import Path +import xmltodict +from aliby.experiment import get_data_lazy +from aliby.io.omero import Argo + + +class ImageLocal: + def __init__(self, path: str): + self.path = path + self.image_id = str(path) + + try: + with TiffFile(fpath) as f: + self.meta = xmltodict.parse(f.ome_metadata)["OME"] + meta = dict() + for dim in self.dimorder: + meta["size_" + dim.lower()] = int( + self.meta["Image"]["Pixels"]["@Size" + dim] + ) + meta["channels"] = [ + x["@Name"] for x in self.meta["Image"]["Pixels"]["Channel"] + ] + meta["name"] = self.meta["Image"]["@Name"] + meta["type"] = self.meta["Image"]["Pixels"]["@Type"] + + self._meta = meta + + except Exception as e: + # TODO implement default meta when no image metadata + print("Could not fetch metadata: {}".format(e)) + + def __enter__(self): + return self + + def __exit__(self, *exc): + return False + + @property + def name(self): + return self._meta["name"] + + @property + def data(self): + return self.get_data_lazy_local() + + @property + def dimorder(self): + """Order of dimensions in image""" + return self.meta["Image"]["Pixels"]["@DimensionOrder"] + + @property + def metadata(self): + return self._meta + + def get_data_lazy_local(self) -> da.Array: + """Return 5D dask array. For lazy-loading local multidimensional tiff files""" + return da.rechunk( + imread(str(self.path))[0], + chunks=(1, 1, 1, self._meta["size_y"], self._meta["size_x"]), + # chunks="auto", + ) + + +class Image(Argo): + """""" + + def __init__(self, image_id, **server_info): + super().__init__(**server_info) + self.image_id = image_id + self._image_wrap = None + + @property + def image_wrap(self): + # TODO check that it is alive/ connected + if self._image_wrap is None: + self._image_wrap = self.conn.getObject("Image", self.image_id) + return self._image_wrap + + # Version with local file processing + def get_data_lazy_local(path: str) -> da.Array: + """Return 5D dask array. For lazy-loading local multidimensional tiff files""" + return da.from_delayed(imread(str(path))[0], shape=()) + + @property + def name(self): + return self.image_wrap.getName() + + @property + def data(self): + return get_data_lazy(self.image_wrap) + + @property + def metadata(self): + meta = dict() + meta["size_x"] = self.image_wrap.getSizeX() + meta["size_y"] = self.image_wrap.getSizeY() + meta["size_z"] = self.image_wrap.getSizeZ() + meta["size_c"] = self.image_wrap.getSizeC() + meta["size_t"] = self.image_wrap.getSizeT() + meta["channels"] = self.image_wrap.getChannelLabels() + meta["name"] = self.image_wrap.getName() + return meta diff --git a/aliby/io/omero.py b/aliby/io/omero.py index 622bebfce20e0d02131fd33c19deca8201e3fd8f..8fa8ed9a52dd6c776e5264fbfda61834a0327065 100644 --- a/aliby/io/omero.py +++ b/aliby/io/omero.py @@ -6,7 +6,6 @@ from agora.io.cells import CellsHDF class Argo: - # TODO use the one in extraction? def __init__( self, host="islay.bio.ed.ac.uk", username="upload", password="***REMOVED***" ): @@ -94,40 +93,6 @@ class Dataset(Argo): return True -class Image(Argo): - def __init__(self, image_id, **server_info): - super().__init__(**server_info) - self.image_id = image_id - self._image_wrap = None - - @property - def image_wrap(self): - # TODO check that it is alive/ connected - if self._image_wrap is None: - self._image_wrap = self.conn.getObject("Image", self.image_id) - return self._image_wrap - - @property - def name(self): - return self.image_wrap.getName() - - @property - def data(self): - return get_data_lazy(self.image_wrap) - - @property - def metadata(self): - meta = dict() - meta["size_x"] = self.image_wrap.getSizeX() - meta["size_y"] = self.image_wrap.getSizeY() - meta["size_z"] = self.image_wrap.getSizeZ() - meta["size_c"] = self.image_wrap.getSizeC() - meta["size_t"] = self.image_wrap.getSizeT() - meta["channels"] = self.image_wrap.getChannelLabels() - meta["name"] = self.image_wrap.getName() - return meta - - class Cells(CellsHDF): def __init__(self, filename): file = h5py.File(filename, "r") diff --git a/poetry.lock b/poetry.lock index 2d334d874244a31aee3e8df3939d2aa3a044cda9..fdd597af1e7a44443d77d80c16858e992b3fd2a4 100644 --- a/poetry.lock +++ b/poetry.lock @@ -78,7 +78,7 @@ python-versions = "*" [[package]] name = "aliby-agora" -version = "0.2.22" +version = "0.2.25" description = "A gathering of shared utilities for the Swain Lab image processing pipeline." category = "main" optional = false @@ -94,14 +94,16 @@ PyYAML = ">=6.0,<7.0" [[package]] name = "aliby-baby" -version = "0.1.7" +version = "0.1.8" description = "Birth Annotator for Budding Yeast" category = "main" optional = false python-versions = ">=3.7.1,<3.11" [package.dependencies] +colorama = ">=0.4.4,<0.5.0" imageio = "2.8.0" +keras-tuner = "1.0.1" matplotlib = ">=3.5.1,<4.0.0" numpy = ">=1.16.0,<2.0.0" pandas = ">=1.1.4" @@ -116,18 +118,20 @@ xgboost = "1.4.2" [[package]] name = "aliby-post" -version = "0.1.21" +version = "0.1.25" description = "Post-processing tools for aliby pipeline." category = "main" optional = false python-versions = ">=3.7.1,<3.11" [package.dependencies] -aliby-agora = ">=0.2.22,<0.3.0" +aliby-agora = ">=0.2.25,<0.3.0" catch22 = ">=0.2.0,<0.3.0" +gaussianprocessderivatives = ">=0.1.2,<0.2.0" leidenalg = ">=0.8.8,<0.9.0" more-itertools = ">=8.12.0,<9.0.0" numpy = ">=1.17.3" +p-tqdm = ">=1.3.3,<2.0.0" pandas = ">=1.1.4,<2.0.0" pathos = ">=0.2.8,<0.3.0" PyYAML = ">=6.0,<7.0" @@ -267,7 +271,7 @@ python-versions = "*" [[package]] name = "charset-normalizer" -version = "2.0.11" +version = "2.0.12" description = "The Real First Universal Charset Detector. Open, modern and actively maintained alternative to Chardet." category = "main" optional = false @@ -278,11 +282,11 @@ unicode_backport = ["unicodedata2"] [[package]] name = "click" -version = "8.0.3" +version = "8.1.0" description = "Composable command line interface toolkit" category = "main" optional = false -python-versions = ">=3.6" +python-versions = ">=3.7" [package.dependencies] colorama = {version = "*", markers = "platform_system == \"Windows\""} @@ -382,7 +386,7 @@ python-versions = ">=2.7, !=3.0.*, !=3.1.*, !=3.2.*, !=3.3.*, !=3.4.*" [[package]] name = "filelock" -version = "3.4.2" +version = "3.6.0" description = "A platform independent file lock." category = "main" optional = false @@ -394,7 +398,7 @@ testing = ["covdefaults (>=1.2.0)", "coverage (>=4)", "pytest (>=4)", "pytest-co [[package]] name = "fonttools" -version = "4.29.1" +version = "4.31.2" description = "Tools to manipulate font files" category = "main" optional = false @@ -423,11 +427,11 @@ python-versions = ">=3.7" [[package]] name = "fsspec" -version = "2022.1.0" +version = "2022.2.0" description = "File-system specification" category = "main" optional = false -python-versions = ">=3.6" +python-versions = ">=3.7" [package.extras] abfs = ["adlfs"] @@ -467,9 +471,22 @@ category = "main" optional = false python-versions = ">=2.7, !=3.0.*, !=3.1.*, !=3.2.*, !=3.3.*" +[[package]] +name = "gaussianprocessderivatives" +version = "0.1.4" +description = "Uses Gaussian processes to smooth data and estimate first- and second-order derivatives" +category = "main" +optional = false +python-versions = ">=3.7.1,<3.11" + +[package.dependencies] +matplotlib = ">=3.5.1,<4.0.0" +numpy = ">=1.16.0,<2.0.0" +scipy = ">=1.7.3,<2.0.0" + [[package]] name = "google-api-core" -version = "2.5.0" +version = "2.7.1" description = "Google API client core library" category = "main" optional = false @@ -533,7 +550,7 @@ six = "*" [[package]] name = "googleapis-common-protos" -version = "1.54.0" +version = "1.56.0" description = "Common protobufs used in Google APIs" category = "main" optional = false @@ -564,7 +581,7 @@ test = ["mock (>=2.0.0)", "pytest (<5.0)"] [[package]] name = "grpcio" -version = "1.43.0" +version = "1.45.0" description = "HTTP/2-based RPC framework" category = "main" optional = false @@ -574,7 +591,7 @@ python-versions = ">=3.6" six = ">=1.5.2" [package.extras] -protobuf = ["grpcio-tools (>=1.43.0)"] +protobuf = ["grpcio-tools (>=1.45.0)"] [[package]] name = "h5py" @@ -641,7 +658,7 @@ python-versions = ">=2.7, !=3.0.*, !=3.1.*, !=3.2.*, !=3.3.*" [[package]] name = "importlib-metadata" -version = "4.10.1" +version = "4.11.3" description = "Read metadata from Python packages" category = "main" optional = false @@ -652,24 +669,24 @@ typing-extensions = {version = ">=3.6.4", markers = "python_version < \"3.8\""} zipp = ">=0.5" [package.extras] -docs = ["sphinx", "jaraco.packaging (>=8.2)", "rst.linker (>=1.9)"] +docs = ["sphinx", "jaraco.packaging (>=9)", "rst.linker (>=1.9)"] perf = ["ipython"] -testing = ["pytest (>=6)", "pytest-checkdocs (>=2.4)", "pytest-flake8", "pytest-cov", "pytest-enabler (>=1.0.1)", "packaging", "pyfakefs", "flufl.flake8", "pytest-perf (>=0.9.2)", "pytest-black (>=0.3.7)", "pytest-mypy", "importlib-resources (>=1.3)"] +testing = ["pytest (>=6)", "pytest-checkdocs (>=2.4)", "pytest-flake8", "pytest-cov", "pytest-enabler (>=1.0.1)", "packaging", "pyfakefs", "flufl.flake8", "pytest-perf (>=0.9.2)", "pytest-black (>=0.3.7)", "pytest-mypy (>=0.9.1)", "importlib-resources (>=1.3)"] [[package]] name = "importlib-resources" -version = "5.4.0" +version = "5.6.0" description = "Read resources from Python packages" category = "main" optional = false -python-versions = ">=3.6" +python-versions = ">=3.7" [package.dependencies] zipp = {version = ">=3.1.0", markers = "python_version < \"3.10\""} [package.extras] -docs = ["sphinx", "jaraco.packaging (>=8.2)", "rst.linker (>=1.9)"] -testing = ["pytest (>=6)", "pytest-checkdocs (>=2.4)", "pytest-flake8", "pytest-cov", "pytest-enabler (>=1.0.1)", "pytest-black (>=0.3.7)", "pytest-mypy"] +docs = ["sphinx", "jaraco.packaging (>=9)", "rst.linker (>=1.9)"] +testing = ["pytest (>=6)", "pytest-checkdocs (>=2.4)", "pytest-flake8", "pytest-cov", "pytest-enabler (>=1.0.1)", "pytest-black (>=0.3.7)", "pytest-mypy (>=0.9.1)"] [[package]] name = "iniconfig" @@ -681,11 +698,11 @@ python-versions = "*" [[package]] name = "jinja2" -version = "3.0.3" +version = "3.1.1" description = "A very fast and expressive template engine." category = "dev" optional = false -python-versions = ">=3.6" +python-versions = ">=3.7" [package.dependencies] MarkupSafe = ">=2.0" @@ -752,24 +769,49 @@ image = ["scipy (>=0.14)", "Pillow (>=5.2.0)"] pep8 = ["flake8"] tests = ["pandas", "pillow", "tensorflow (==1.7)", "keras", "pytest", "pytest-xdist", "pytest-cov"] +[[package]] +name = "keras-tuner" +version = "1.0.1" +description = "Hypertuner for Keras" +category = "main" +optional = false +python-versions = "*" + +[package.dependencies] +colorama = "*" +future = "*" +numpy = "*" +requests = "*" +scikit-learn = "*" +scipy = "*" +tabulate = "*" +terminaltables = "*" +tqdm = "*" + +[package.extras] +tests = ["pytest", "flake8", "mock", "portpicker", "pytest-xdist", "pytest-cov"] + [[package]] name = "kiwisolver" -version = "1.3.2" +version = "1.4.2" description = "A fast implementation of the Cassowary constraint solver" category = "main" optional = false python-versions = ">=3.7" +[package.dependencies] +typing-extensions = {version = "*", markers = "python_version < \"3.8\""} + [[package]] name = "leidenalg" -version = "0.8.8" +version = "0.8.9" description = "Leiden is a general algorithm for methods of community detection in large networks." category = "main" optional = false python-versions = "*" [package.dependencies] -python-igraph = ">=0.9.0" +igraph = ">=0.9.0,<0.10" [[package]] name = "locket" @@ -795,11 +837,11 @@ testing = ["coverage", "pyyaml"] [[package]] name = "markupsafe" -version = "2.0.1" +version = "2.1.1" description = "Safely add untrusted strings to HTML/XML markup." category = "dev" optional = false -python-versions = ">=3.6" +python-versions = ">=3.7" [[package]] name = "matplotlib" @@ -927,7 +969,7 @@ signedtoken = ["cryptography (>=3.0.0)", "pyjwt (>=2.0.0,<3)"] [[package]] name = "omero-py" -version = "5.11.0" +version = "5.11.1" description = "Python bindings to the OMERO.blitz server" category = "main" optional = false @@ -976,7 +1018,7 @@ numpy = ">=1.13.3" [[package]] name = "opencv-python" -version = "4.5.5.62" +version = "4.5.5.64" description = "Wrapper package for OpenCV python bindings." category = "main" optional = false @@ -1003,6 +1045,19 @@ numpy = ">=1.7" docs = ["sphinx (==1.2.3)", "sphinxcontrib-napoleon", "sphinx-rtd-theme", "numpydoc"] tests = ["pytest", "pytest-cov", "pytest-pep8"] +[[package]] +name = "p-tqdm" +version = "1.3.3" +description = "Parallel processing with progress bars" +category = "main" +optional = false +python-versions = "*" + +[package.dependencies] +pathos = "*" +six = "*" +tqdm = "*" + [[package]] name = "packaging" version = "21.3" @@ -1109,7 +1164,7 @@ python-versions = ">=3.7" [[package]] name = "platformdirs" -version = "2.5.0" +version = "2.5.1" description = "A small Python module for determining appropriate platform-specific dirs, e.g. a \"user data dir\"." category = "dev" optional = false @@ -1304,26 +1359,9 @@ python-versions = "!=3.0.*,!=3.1.*,!=3.2.*,>=2.7" [package.dependencies] six = ">=1.5" -[[package]] -name = "python-igraph" -version = "0.9.9" -description = "High performance graph data structures and algorithms (legacy package)" -category = "main" -optional = false -python-versions = ">=3.6" - -[package.dependencies] -igraph = "0.9.9" - -[package.extras] -cairo = ["cairocffi (>=1.2.0)"] -matplotlib = ["matplotlib (>=3.3.0)"] -plotly = ["plotly (>=5.3.0)"] -plotting = ["cairocffi (>=1.2.0)"] - [[package]] name = "pytz" -version = "2021.3" +version = "2022.1" description = "World timezone definitions, modern and historical" category = "main" optional = false @@ -1331,7 +1369,7 @@ python-versions = "*" [[package]] name = "pywavelets" -version = "1.2.0" +version = "1.3.0" description = "PyWavelets, wavelet transform module" category = "main" optional = false @@ -1399,16 +1437,18 @@ tune = ["pandas", "tabulate", "tensorboardx"] [[package]] name = "redis" -version = "4.1.3" +version = "4.2.0" description = "Python client for Redis database and key-value store" category = "main" optional = false python-versions = ">=3.6" [package.dependencies] +async-timeout = ">=4.0.2" deprecated = ">=1.2.3" importlib-metadata = {version = ">=1.0", markers = "python_version < \"3.8\""} packaging = ">=20.4" +typing-extensions = "*" [package.extras] hiredis = ["hiredis (>=1.0.0)"] @@ -1568,7 +1608,7 @@ python-versions = "*" [[package]] name = "sphinx" -version = "4.4.0" +version = "4.5.0" description = "Python documentation generator" category = "dev" optional = false @@ -1702,7 +1742,7 @@ werkzeug = ">=0.11.15" [[package]] name = "tensorboardx" -version = "2.4.1" +version = "2.5" description = "TensorBoardX lets you watch Tensors Flow without Tensorflow" category = "main" optional = false @@ -1711,6 +1751,7 @@ python-versions = "*" [package.dependencies] numpy = "*" protobuf = ">=3.8.0" +six = "*" [[package]] name = "tensorflow" @@ -1754,6 +1795,14 @@ category = "main" optional = false python-versions = "*" +[[package]] +name = "terminaltables" +version = "3.1.10" +description = "Generate simple tables in terminals from a nested list of strings." +category = "main" +optional = false +python-versions = ">=2.6" + [[package]] name = "texttable" version = "1.6.4" @@ -1802,7 +1851,7 @@ python-versions = ">=3.5" [[package]] name = "tqdm" -version = "4.62.3" +version = "4.63.1" description = "Fast, Extensible Progress Meter" category = "main" optional = false @@ -1840,7 +1889,7 @@ python-versions = ">=3.6" [[package]] name = "typing-extensions" -version = "4.0.1" +version = "4.1.1" description = "Backported and Experimental Type Hints for Python 3.6+" category = "main" optional = false @@ -1848,31 +1897,31 @@ python-versions = ">=3.6" [[package]] name = "urllib3" -version = "1.26.8" +version = "1.26.9" description = "HTTP library with thread-safe connection pooling, file post, and more." category = "main" optional = false python-versions = ">=2.7, !=3.0.*, !=3.1.*, !=3.2.*, !=3.3.*, !=3.4.*, <4" [package.extras] -brotli = ["brotlipy (>=0.6.0)"] +brotli = ["brotlicffi (>=0.8.0)", "brotli (>=1.0.9)", "brotlipy (>=0.6.0)"] secure = ["pyOpenSSL (>=0.14)", "cryptography (>=1.3.4)", "idna (>=2.0.0)", "certifi", "ipaddress"] socks = ["PySocks (>=1.5.6,!=1.5.7,<2.0)"] [[package]] name = "werkzeug" -version = "2.0.3" +version = "2.1.0" description = "The comprehensive WSGI web application library." category = "main" optional = false -python-versions = ">=3.6" +python-versions = ">=3.7" [package.extras] watchdog = ["watchdog"] [[package]] name = "wrapt" -version = "1.13.3" +version = "1.14.0" description = "Module for decorators, wrappers and monkey patching." category = "main" optional = false @@ -1897,6 +1946,14 @@ pandas = ["pandas"] plotting = ["graphviz", "matplotlib"] scikit-learn = ["scikit-learn"] +[[package]] +name = "xmltodict" +version = "0.13.0" +description = "Makes working with XML feel like you are working with JSON" +category = "main" +optional = false +python-versions = ">=3.4" + [[package]] name = "yarl" version = "1.7.2" @@ -1933,7 +1990,7 @@ testing = ["pytest (>=6)", "pytest-checkdocs (>=2.4)", "pytest-flake8", "pytest- [metadata] lock-version = "1.1" python-versions = ">=3.7.1,<3.11" -content-hash = "9243e6acc7477341296d163934bc99e0af5fae3f12adda96c5daa057a1cd33a5" +content-hash = "002101f6087ef03167c900419fe42edafdc0d4c0e43fc392ecfdc7ea852e29b8" [metadata.files] absl-py = [ @@ -2031,16 +2088,16 @@ alabaster = [ {file = "alabaster-0.7.12.tar.gz", hash = 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