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docs/run-notes/19973_run_notes.md

+# Run 19973
+
+## Pre-run info
+
+### Other family members on previous runs
+* 453234, parents (134865 and 134864) are on this run, proband was on run 19690 (132524)
+* 414996, proband (135612) and father (117093) are on this run, mother was on run 18203 (121256)
+* 457300, proband (134113) and mother (134114) are on this run, father was on run 19875 (134116)
+
+### Singletons
+* 457456 (FFPE tissue, might fail)
+* 480173
+
+### Duos (for singleton analysis)
+* 457600 (proband: 134484)
+* 458099 (proband: 129313)
+
+### Priority
+* 414996, 480290, 457300, 481401, 481293
+
+## QC summary
+
+* 457546
+  * 135245 - proband - 0.3M reads, contamination 14%, C>T/G>A deamination artefacts (probably 30% of variants) - fail, do not proceed with prioritization
+* 457300
+  * 134113 - proband - 27X - low but usable
+  * 134116 - father - 33X - low but usable, failed per-tile sequence quality in FastQC (unusual)
+* 481089
+  * 105222 - mother - 42X - borderline low, fine to analyze
+* 473144
+  * 135054 - father - 45X - borderline low, fine to analyze
+* 414996
+  * 117093 - father - contamination 10% - proceed with prioritization with caution