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Commit be512736 authored by not populated not populated's avatar not populated not populated
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Links rather than text copy for config files and bcbio templates

parent 5262d001
1 merge request!3Ultra 2 SOP/doc updates
...@@ -78,55 +78,11 @@ The project working directories will be in the folder `/home/u035/u035/shared/an ...@@ -78,55 +78,11 @@ The project working directories will be in the folder `/home/u035/u035/shared/an
## Project configuration ## Project configuration
A configuration script sets environment variables common to scripts used in this SOP. This is stored at `/home/u035/u035/shared/scripts/trio_whole_exome_config.sh`. A [configuration script](../trio_whole_exome_config.sh) sets environment variables common to scripts used in this SOP.
```
#!/usr/bin/bash
#
# Basic configuration options for trio WES pipeline
#
BASE=/home/u035/u035/shared
SCRIPTS=$BASE/scripts
BCBIO_TEMPLATE=$SCRIPTS/trio_whole_exome_bcbio_template.yaml
TARGET=$BASE/resources/Twist_Exome_RefSeq_targets_hg38.plus15bp.bed
DOWNLOAD_DIR=$BASE/data
REFERENCE_GENOME=$BASE/software/bcbio/genomes/Hsapiens/hg38/seq/hg38.fa
PARAMS_DIR=$BASE/analysis/params
READS_DIR=$BASE/analysis/reads
CONFIG_DIR=$BASE/analysis/config
WORK_DIR=$BASE/analysis/work
OUTPUT_DIR=$BASE/analysis/results
export PATH=$BASE/software/bcbio/tools/bin:$PATH
````
## Template for bcbio configuration ## Template for bcbio configuration
Bcbio requires a template file in YAML format to define the procedures run in the pipeline. The template for this project is stored at `/home/u035/u035/shared/scripts/trio_whole_exome_bcbio_template.yaml`. Bcbio requires a [template file in YAML format](../trio_whole_exome_bcbio_template.yaml). to define the procedures run in the pipeline. Runs from the Edinburgh CRF have a [slightly different template file](../trio_whole_exome_bcbio_crf_template.yaml) which adds read trimming parameters specific to NextSeq 2000 output.
```
details:
- algorithm:
platform: illumina
quality_format: standard
aligner: bwa
mark_duplicates: true
realign: false
recalibrate: true
effects: vep
effects_transcripts: all
variantcaller: gatk-haplotype
indelcaller: false
remove_lcr: true
tools_on:
- vep_splicesite_annotations
analysis: variant2
genome_build: hg38
upload:
dir: /home/u035/u035/shared/results
```
## Output ## Output
......
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