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Commit fbbccaef authored by ameyner2's avatar ameyner2
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Instructions for manual installation of gnomAD.

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......@@ -21,6 +21,27 @@ python bcbio_nextgen_install.py /home/u035/project/software/bcbio \
--datatarget vep --datatarget dbnsfp --cores 32 &> bcbio_install_YYYYMMDDhhmm.log
```
The bcbio gnomAD installation was failing because the software *vt* had problems. Solution: replace vt with version from bcbio-1.2.0. See https://github.com/bcbio/bcbio-nextgen/issues/3327 and https://github.com/bcbio/bcbio-nextgen/issues/3328.
```
cd /home/u035/project/software/bcbio/anaconda/bin
mv vt vt.bcbio-1.2.3
scp ameyner2@eddie.ecdf.ed.ac.uk:/exports/igmm/software/pkg/el7/apps/bcbio/1.2.0/bin/vt ./
chmod a+x vt
cd ../../genomes/Hsapiens/hg38/txtmp/
qsub /home/u035/project/scripts/bcbio_gnomad_install.sh
```
This will take a few days (4-6) to run. When complete:
```
cd ../../genomes/Hsapiens/hg38/txtmp/
mv variation/gnomad* ../variation/
```
Now restart the installation as above.
Put a fake file name for genotype2phenotype field in genomes/hg38/seq/hg38-resources.yaml
Increase JVM memory for GATK in galaxy/bcbio_system.yaml
......
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