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Swain Lab
aliby
alibylite
Commits
31f64e47
Commit
31f64e47
authored
2 years ago
by
pswain
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removed dict keyword from traps.py
parent
31e767d4
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1 changed file
aliby/tile/traps.py
+15
-14
15 additions, 14 deletions
aliby/tile/traps.py
with
15 additions
and
14 deletions
aliby/tile/traps.py
+
15
−
14
View file @
31f64e47
...
...
@@ -34,7 +34,7 @@ def segment_traps(
square_size
=
3
,
min_frac_tilesize
=
0.2
,
max_frac_tilesize
=
0.8
,
identify_traps_kwargs
=
{}
,
**
identify_traps_kwargs
,
):
"""
Uses an entropy filter and Otsu thresholding to find a trap template,
...
...
@@ -58,8 +58,8 @@ def segment_traps(
max_frac_tilesize: float (optional)
Used to determine bounds on the major axis length of regions
suspected of containing traps.
identify_traps_kwargs:
dictionary
Passed to identify_trap_locations
function
identify_traps_kwargs:
Passed to identify_trap_locations
Returns
-------
...
...
@@ -115,14 +115,14 @@ def segment_traps(
# make a template using the best trap in the image
template
=
image
[
half_floor
(
x
,
tile_size
)
:
half_ceil
(
x
,
tile_size
),
half_floor
(
y
,
tile_size
)
:
half_ceil
(
y
,
tile_size
),
half_floor
(
x
,
tile_size
)
:
half_ceil
(
x
,
tile_size
),
half_floor
(
y
,
tile_size
)
:
half_ceil
(
y
,
tile_size
),
]
# make candidate templates from the other traps found
candidate_templates
=
[
image
[
half_floor
(
x
,
tile_size
)
:
half_ceil
(
x
,
tile_size
),
half_floor
(
y
,
tile_size
)
:
half_ceil
(
y
,
tile_size
),
half_floor
(
x
,
tile_size
)
:
half_ceil
(
x
,
tile_size
),
half_floor
(
y
,
tile_size
)
:
half_ceil
(
y
,
tile_size
),
]
for
x
,
y
in
centroids
]
...
...
@@ -187,7 +187,7 @@ def identify_trap_locations(
coordinates: an array of pairs of integers
"""
if
trap_size
is
None
:
trap_size
=
trap_template
.
shape
[
0
]
trap_size
=
trap_template
.
shape
[
0
]
# careful: the image is float16!
img
=
transform
.
rescale
(
image
.
astype
(
float
),
downscale
)
template
=
transform
.
rescale
(
trap_template
,
downscale
)
...
...
@@ -245,10 +245,12 @@ def identify_trap_locations(
)
return
coordinates
###############################################################
# functions below here do not appear to be used any more
###############################################################
def
stretch_image
(
image
):
image
=
((
image
-
image
.
min
())
/
(
image
.
max
()
-
image
.
min
()))
*
255
minval
=
np
.
percentile
(
image
,
2
)
...
...
@@ -258,7 +260,6 @@ def stretch_image(image):
return
image
def
get_tile_shapes
(
x
,
tile_size
,
max_shape
):
half_size
=
tile_size
//
2
xmin
=
int
(
x
[
0
]
-
half_size
)
...
...
@@ -392,7 +393,7 @@ def get_trap_timelapse_omero(
ch
=
channels
.
index
(
c
)
tp
=
times
.
tolist
().
index
(
t
)
z_pos
=
z_positions
.
index
(
z
)
timelapse
[
ch
,
tp
,
x
[
0
]
:
x
[
1
],
y
[
0
]
:
y
[
1
],
z_pos
]
=
image
timelapse
[
ch
,
tp
,
x
[
0
]:
x
[
1
],
y
[
0
]:
y
[
1
],
z_pos
]
=
image
# for x in timelapse: # By channel
# np.nan_to_num(x, nan=np.nanmedian(x), copy=False)
...
...
@@ -475,8 +476,8 @@ def get_tile(shape, center, raw_expt, ch, t, z):
tile
[
:,
:,
(
r_xmin
-
xmin
)
:
(
r_xmax
-
xmin
),
(
r_ymin
-
ymin
)
:
(
r_ymax
-
ymin
),
(
r_xmin
-
xmin
):
(
r_xmax
-
xmin
),
(
r_ymin
-
ymin
):
(
r_ymax
-
ymin
),
:,
]
=
raw_expt
[
ch
,
t
,
r_xmin
:
r_xmax
,
r_ymin
:
r_ymax
,
z
]
# fill_val = np.nanmedian(tile)
...
...
@@ -526,7 +527,7 @@ def get_traps_timepoint(
):
ch
=
channels
.
index
(
c
)
z_pos
=
z_positions
.
index
(
z
)
traps
[
trap_id
,
ch
,
0
,
x
[
0
]
:
x
[
1
],
y
[
0
]
:
y
[
1
],
z_pos
]
=
image
traps
[
trap_id
,
ch
,
0
,
x
[
0
]:
x
[
1
],
y
[
0
]:
y
[
1
],
z_pos
]
=
image
for
x
in
traps
:
# By channel
np
.
nan_to_num
(
x
,
nan
=
np
.
nanmedian
(
x
),
copy
=
False
)
return
traps
...
...
@@ -538,7 +539,7 @@ def centre(img, percentage=0.3):
cropy
=
int
(
np
.
ceil
(
y
*
percentage
))
startx
=
int
(
x
//
2
-
(
cropx
//
2
))
starty
=
int
(
y
//
2
-
(
cropy
//
2
))
return
img
[
starty
:
starty
+
cropy
,
startx
:
startx
+
cropx
]
return
img
[
starty
:
starty
+
cropy
,
startx
:
startx
+
cropx
]
def
align_timelapse_images
(
...
...
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