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Swain Lab
aliby
alibylite
Commits
c78d0427
Commit
c78d0427
authored
2 years ago
by
Alán Muñoz
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change(aliby): move omero Image to omero.py
parent
1c668ecc
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2 changed files
src/aliby/io/image.py
+2
-153
2 additions, 153 deletions
src/aliby/io/image.py
src/aliby/io/omero.py
+157
-1
157 additions, 1 deletion
src/aliby/io/omero.py
with
159 additions
and
154 deletions
src/aliby/io/image.py
+
2
−
153
View file @
c78d0427
...
...
@@ -6,29 +6,11 @@ from datetime import datetime
from
pathlib
import
Path
,
PosixPath
import
dask.array
as
da
import
numpy
as
np
import
xmltodict
from
dask
import
delayed
from
dask.array.image
import
imread
from
omero.model
import
enums
as
omero_enums
from
tifffile
import
TiffFile
from
yaml
import
safe_load
from
agora.io.bridge
import
BridgeH5
from
agora.io.metadata
import
dir_to_meta
from
aliby.io.omero
import
BridgeOmero
# convert OMERO definitions into numpy types
PIXEL_TYPES
=
{
omero_enums
.
PixelsTypeint8
:
np
.
int8
,
omero_enums
.
PixelsTypeuint8
:
np
.
uint8
,
omero_enums
.
PixelsTypeint16
:
np
.
int16
,
omero_enums
.
PixelsTypeuint16
:
np
.
uint16
,
omero_enums
.
PixelsTypeint32
:
np
.
int32
,
omero_enums
.
PixelsTypeuint32
:
np
.
uint32
,
omero_enums
.
PixelsTypefloat
:
np
.
float32
,
omero_enums
.
PixelsTypedouble
:
np
.
float64
,
}
def
get_image_class
(
source
:
t
.
Union
[
str
,
int
,
t
.
Dict
[
str
,
str
],
PosixPath
]):
...
...
@@ -36,6 +18,8 @@ def get_image_class(source: t.Union[str, int, t.Dict[str, str], PosixPath]):
Wrapper to pick the appropiate Image class depending on the source of data.
"""
if
isinstance
(
source
,
int
):
from
aliby.io.omero
import
Image
instatiator
=
Image
elif
isinstance
(
source
,
dict
)
or
(
isinstance
(
source
,
(
str
,
PosixPath
))
and
Path
(
source
).
is_dir
()
...
...
@@ -262,138 +246,3 @@ class ImageDir(BaseLocalImage):
return
[
k
.
split
(
"
_
"
)[
-
1
]
for
k
in
self
.
_meta
.
keys
()
if
k
.
startswith
(
"
size
"
)
]
class
Image
(
BridgeOmero
):
"""
Loads images from OMERO and gives access to the data and metadata.
"""
def
__init__
(
self
,
image_id
:
int
,
**
server_info
):
"""
Establishes the connection to the OMERO server via the Argo
base class.
Parameters
----------
image_id: integer
server_info: dictionary
Specifies the host, username, and password as strings
"""
self
.
ome_id
=
image_id
super
().
__init__
(
**
server_info
)
def
init_interface
(
self
,
ome_id
:
int
):
self
.
set_id
(
ome_id
)
self
.
ome_class
=
self
.
conn
.
getObject
(
"
Image
"
,
ome_id
)
@classmethod
def
from_h5
(
cls
,
filepath
:
t
.
Union
[
str
,
PosixPath
],
):
"""
Instatiate Image from a hdf5 file.
Parameters
----------
cls : Image
Image class
filepath : t.Union[str, PosixPath]
Location of hdf5 file.
Examples
--------
FIXME: Add docs.
"""
# metadata = load_attributes(filepath)
bridge
=
BridgeH5
(
filepath
)
image_id
=
bridge
.
meta_h5
[
"
image_id
"
]
# server_info = safe_load(bridge.meta_h5["parameters"])["general"][
# "server_info"
# ]
return
cls
(
image_id
,
**
cls
.
server_info_from_h5
(
filepath
))
@property
def
name
(
self
):
return
self
.
ome_class
.
getName
()
@property
def
data
(
self
):
return
get_data_lazy
(
self
.
ome_class
)
@property
def
metadata
(
self
):
"""
Store metadata saved in OMERO: image size, number of time points,
labels of channels, and image name.
"""
meta
=
dict
()
meta
[
"
size_x
"
]
=
self
.
ome_class
.
getSizeX
()
meta
[
"
size_y
"
]
=
self
.
ome_class
.
getSizeY
()
meta
[
"
size_z
"
]
=
self
.
ome_class
.
getSizeZ
()
meta
[
"
size_c
"
]
=
self
.
ome_class
.
getSizeC
()
meta
[
"
size_t
"
]
=
self
.
ome_class
.
getSizeT
()
meta
[
"
channels
"
]
=
self
.
ome_class
.
getChannelLabels
()
meta
[
"
name
"
]
=
self
.
ome_class
.
getName
()
return
meta
class
UnsafeImage
(
Image
):
"""
Loads images from OMERO and gives access to the data and metadata.
This class is a temporary solution while we find a way to use
context managers inside napari. It risks resulting in zombie connections
and producing freezes in an OMERO server.
"""
def
__init__
(
self
,
image_id
,
**
server_info
):
"""
Establishes the connection to the OMERO server via the Argo
base class.
Parameters
----------
image_id: integer
server_info: dictionary
Specifies the host, username, and password as strings
"""
super
().
__init__
(
image_id
,
**
server_info
)
self
.
create_gate
()
self
.
init_wrapper
()
@property
def
data
(
self
):
try
:
return
get_data_lazy
(
self
.
ome_class
)
except
Exception
as
e
:
print
(
f
"
ERROR: Failed fetching image from server:
{
e
}
"
)
self
.
conn
.
connect
(
False
)
def
get_data_lazy
(
image
)
->
da
.
Array
:
"""
Get 5D dask array, with delayed reading from OMERO image.
"""
nt
,
nc
,
nz
,
ny
,
nx
=
[
getattr
(
image
,
f
"
getSize
{
x
}
"
)()
for
x
in
"
TCZYX
"
]
pixels
=
image
.
getPrimaryPixels
()
dtype
=
PIXEL_TYPES
.
get
(
pixels
.
getPixelsType
().
value
,
None
)
# using dask
get_plane
=
delayed
(
lambda
idx
:
pixels
.
getPlane
(
*
idx
))
def
get_lazy_plane
(
zct
):
return
da
.
from_delayed
(
get_plane
(
zct
),
shape
=
(
ny
,
nx
),
dtype
=
dtype
)
# 5D stack: TCZXY
t_stacks
=
[]
for
t
in
range
(
nt
):
c_stacks
=
[]
for
c
in
range
(
nc
):
z_stack
=
[]
for
z
in
range
(
nz
):
z_stack
.
append
(
get_lazy_plane
((
z
,
c
,
t
)))
c_stacks
.
append
(
da
.
stack
(
z_stack
))
t_stacks
.
append
(
da
.
stack
(
c_stacks
))
return
da
.
stack
(
t_stacks
)
This diff is collapsed.
Click to expand it.
src/aliby/io/omero.py
+
157
−
1
View file @
c78d0427
"""
Tools to manage I/O using a remote OMERO server.
"""
import
re
import
typing
as
t
from
abc
import
abstractmethod
from
pathlib
import
PosixPath
import
dask.array
as
da
import
numpy
as
np
import
omero
from
dask
import
delayed
from
omero.gateway
import
BlitzGateway
from
omero.model
import
enums
as
omero_enums
from
yaml
import
safe_load
from
agora.io.bridge
import
BridgeH5
from
aliby.io.omero
import
BridgeOmero
# convert OMERO definitions into numpy types
PIXEL_TYPES
=
{
omero_enums
.
PixelsTypeint8
:
np
.
int8
,
omero_enums
.
PixelsTypeuint8
:
np
.
uint8
,
omero_enums
.
PixelsTypeint16
:
np
.
int16
,
omero_enums
.
PixelsTypeuint16
:
np
.
uint16
,
omero_enums
.
PixelsTypeint32
:
np
.
int32
,
omero_enums
.
PixelsTypeuint32
:
np
.
uint32
,
omero_enums
.
PixelsTypefloat
:
np
.
float32
,
omero_enums
.
PixelsTypedouble
:
np
.
float64
,
}
class
BridgeOmero
:
...
...
@@ -195,7 +216,7 @@ class Dataset(BridgeOmero):
def
cache_logs
(
self
,
root_dir
):
valid_suffixes
=
(
"
txt
"
,
"
log
"
)
for
name
,
annotation
in
self
.
files
.
items
():
for
_
,
annotation
in
self
.
files
.
items
():
filepath
=
root_dir
/
annotation
.
getFileName
().
replace
(
"
/
"
,
"
_
"
)
if
(
any
([
str
(
filepath
).
endswith
(
suff
)
for
suff
in
valid_suffixes
])
...
...
@@ -234,3 +255,138 @@ class Dataset(BridgeOmero):
return
cls
(
bridge
.
meta_h5
[
k
],
**
cls
.
server_info_from_h5
(
filepath
)
)
class
Image
(
BridgeOmero
):
"""
Loads images from OMERO and gives access to the data and metadata.
"""
def
__init__
(
self
,
image_id
:
int
,
**
server_info
):
"""
Establishes the connection to the OMERO server via the Argo
base class.
Parameters
----------
image_id: integer
server_info: dictionary
Specifies the host, username, and password as strings
"""
self
.
ome_id
=
image_id
super
().
__init__
(
**
server_info
)
def
init_interface
(
self
,
ome_id
:
int
):
self
.
set_id
(
ome_id
)
self
.
ome_class
=
self
.
conn
.
getObject
(
"
Image
"
,
ome_id
)
@classmethod
def
from_h5
(
cls
,
filepath
:
t
.
Union
[
str
,
PosixPath
],
):
"""
Instatiate Image from a hdf5 file.
Parameters
----------
cls : Image
Image class
filepath : t.Union[str, PosixPath]
Location of hdf5 file.
Examples
--------
FIXME: Add docs.
"""
# metadata = load_attributes(filepath)
bridge
=
BridgeH5
(
filepath
)
image_id
=
bridge
.
meta_h5
[
"
image_id
"
]
# server_info = safe_load(bridge.meta_h5["parameters"])["general"][
# "server_info"
# ]
return
cls
(
image_id
,
**
cls
.
server_info_from_h5
(
filepath
))
@property
def
name
(
self
):
return
self
.
ome_class
.
getName
()
@property
def
data
(
self
):
return
get_data_lazy
(
self
.
ome_class
)
@property
def
metadata
(
self
):
"""
Store metadata saved in OMERO: image size, number of time points,
labels of channels, and image name.
"""
meta
=
dict
()
meta
[
"
size_x
"
]
=
self
.
ome_class
.
getSizeX
()
meta
[
"
size_y
"
]
=
self
.
ome_class
.
getSizeY
()
meta
[
"
size_z
"
]
=
self
.
ome_class
.
getSizeZ
()
meta
[
"
size_c
"
]
=
self
.
ome_class
.
getSizeC
()
meta
[
"
size_t
"
]
=
self
.
ome_class
.
getSizeT
()
meta
[
"
channels
"
]
=
self
.
ome_class
.
getChannelLabels
()
meta
[
"
name
"
]
=
self
.
ome_class
.
getName
()
return
meta
class
UnsafeImage
(
Image
):
"""
Loads images from OMERO and gives access to the data and metadata.
This class is a temporary solution while we find a way to use
context managers inside napari. It risks resulting in zombie connections
and producing freezes in an OMERO server.
"""
def
__init__
(
self
,
image_id
,
**
server_info
):
"""
Establishes the connection to the OMERO server via the Argo
base class.
Parameters
----------
image_id: integer
server_info: dictionary
Specifies the host, username, and password as strings
"""
super
().
__init__
(
image_id
,
**
server_info
)
self
.
create_gate
()
self
.
init_wrapper
()
@property
def
data
(
self
):
try
:
return
get_data_lazy
(
self
.
ome_class
)
except
Exception
as
e
:
print
(
f
"
ERROR: Failed fetching image from server:
{
e
}
"
)
self
.
conn
.
connect
(
False
)
def
get_data_lazy
(
image
)
->
da
.
Array
:
"""
Get 5D dask array, with delayed reading from OMERO image.
"""
nt
,
nc
,
nz
,
ny
,
nx
=
[
getattr
(
image
,
f
"
getSize
{
x
}
"
)()
for
x
in
"
TCZYX
"
]
pixels
=
image
.
getPrimaryPixels
()
dtype
=
PIXEL_TYPES
.
get
(
pixels
.
getPixelsType
().
value
,
None
)
# using dask
get_plane
=
delayed
(
lambda
idx
:
pixels
.
getPlane
(
*
idx
))
def
get_lazy_plane
(
zct
):
return
da
.
from_delayed
(
get_plane
(
zct
),
shape
=
(
ny
,
nx
),
dtype
=
dtype
)
# 5D stack: TCZXY
t_stacks
=
[]
for
t
in
range
(
nt
):
c_stacks
=
[]
for
c
in
range
(
nc
):
z_stack
=
[]
for
z
in
range
(
nz
):
z_stack
.
append
(
get_lazy_plane
((
z
,
c
,
t
)))
c_stacks
.
append
(
da
.
stack
(
z_stack
))
t_stacks
.
append
(
da
.
stack
(
c_stacks
))
return
da
.
stack
(
t_stacks
)
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