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Swain Lab
wela
Commits
9ff355a4
Commit
9ff355a4
authored
10 months ago
by
pswain
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change(imageviewer): one class with classmethods
parent
4dd9423f
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src/wela/imageviewer.py
+103
-62
103 additions, 62 deletions
src/wela/imageviewer.py
with
103 additions
and
62 deletions
src/wela/imageviewer.py
+
103
−
62
View file @
9ff355a4
import
typing
as
t
from
abc
import
ABC
from
pathlib
import
Path
import
h5py
...
...
@@ -12,25 +10,57 @@ from aliby.io.omero import UnsafeImage as OImage
from
aliby.tile.tiler
import
Tiler
def
colormap
(
channel
):
"""
Find default colormap.
"""
if
"
GFP
"
in
channel
:
colormap
=
"
green
"
elif
"
Cherry
"
in
channel
or
"
RFP
"
in
channel
:
colormap
=
"
red
"
else
:
colormap
=
"
gray
"
return
colormap
class
ImageViewer
:
def
__init__
(
self
,
h5file
):
self
.
h5file_path
=
Path
(
h5file
)
print
(
f
"
Viewing
{
str
(
self
.
h5file_path
)
}
"
)
self
.
full
=
{}
class
BaseImageViewer
(
ABC
):
"""
Base class for all ImageViewers.
"""
@classmethod
def
local
(
cls
,
h5file
:
str
,
image_file
:
str
):
"""
View images from local files.
def
__init__
(
self
,
h5file_path
):
"""
Initialise from a Path to a h5 file.
"""
self
.
h5file_path
=
h5file_path
print
(
f
"
Viewing
{
str
(
h5file_path
)
}
"
)
self
.
full
=
{}
Files are either zarr or organised in directories.
"""
iv
=
cls
(
h5file
)
image_file_path
=
Path
(
image_file
)
if
iv
.
h5file_path
.
exists
()
and
image_file_path
.
exists
():
with
dispatch_image
(
image_file_path
)(
image_file_path
)
as
image
:
tiler
=
Tiler
.
from_h5
(
image
,
iv
.
h5file_path
)
cells
=
Cells
.
from_source
(
iv
.
h5file_path
)
iv
.
tiler
=
tiler
iv
.
cells
=
cells
return
iv
else
:
if
not
iv
.
h5file_path
.
exists
():
print
(
f
"
Trouble loading
{
h5file
}
.
"
)
if
not
image_file_path
.
exists
():
print
(
f
"
Trouble loading
{
image_file
}
.
"
)
@classmethod
def
remote
(
cls
,
h5file
:
str
,
server_info
:
dict
,
omero_id
:
int
):
"""
View images from OMERO.
"""
iv
=
cls
(
h5file
)
with
h5py
.
File
(
iv
.
h5file_path
,
"
r
"
)
as
f
:
# get image_id from the h5 file
image_id
=
f
.
attrs
.
get
(
"
image_id
"
)
if
image_id
is
None
:
# get image_id from OMERO
with
Dataset
(
omero_id
,
**
server_info
)
as
dataset_om
:
positions
=
dataset_om
.
get_position_ids
()
image_id
=
positions
.
get
(
iv
.
h5file_path
.
name
.
split
(
"
.
"
)[
0
])
if
image_id
is
None
:
print
(
"
Can
'
t find an image.
"
)
else
:
print
(
f
"
Using image ID
{
image_id
}
.
"
)
iv
.
image_id
=
image_id
image
=
OImage
(
image_id
,
**
server_info
)
print
(
"
Connected to OMERO.
"
)
iv
.
tiler
=
Tiler
.
from_h5
(
image
,
iv
.
h5file_path
)
iv
.
cells
=
Cells
.
from_source
(
iv
.
h5file_path
)
return
iv
def
find_traps_with_cells
(
self
,
tpt_end
,
tpt_start
=
0
):
"""
List traps with cells.
"""
...
...
@@ -192,55 +222,66 @@ class BaseImageViewer(ABC):
)
class
LocalImageViewer
(
BaseImageViewer
):
"""
View images from local files.
#
class LocalImageViewer(BaseImageViewer):
#
"""
#
View images from local files.
Files are either zarr or organised in directories.
"""
#
Files are either zarr or organised in directories.
#
"""
def
__init__
(
self
,
h5file
:
str
,
image_file
:
str
):
"""
Initialise using a h5file and a zarr file of images.
"""
h5file_path
=
Path
(
h5file
)
image_file_path
=
Path
(
image_file
)
if
h5file_path
.
exists
()
and
image_file_path
.
exists
():
super
().
__init__
(
h5file_path
)
with
dispatch_image
(
image_file_path
)(
image_file_path
)
as
image
:
self
.
tiler
=
Tiler
.
from_h5
(
image
,
h5file_path
)
self
.
cells
=
Cells
.
from_source
(
h5file_path
)
else
:
if
not
h5file_path
.
exists
():
print
(
f
"
Trouble loading
{
h5file
}
.
"
)
if
not
image_file_path
.
exists
():
print
(
f
"
Trouble loading
{
image_file
}
.
"
)
#
def __init__(self, h5file: str, image_file: str):
#
"""Initialise using a h5file and a zarr file of images."""
#
h5file_path = Path(h5file)
#
image_file_path = Path(image_file)
#
if h5file_path.exists() and image_file_path.exists():
#
super().__init__(h5file_path)
#
with dispatch_image(image_file_path)(image_file_path) as image:
#
self.tiler = Tiler.from_h5(image, h5file_path)
#
self.cells = Cells.from_source(h5file_path)
#
else:
#
if not h5file_path.exists():
#
print(f" Trouble loading {h5file}.")
#
if not image_file_path.exists():
#
print(f" Trouble loading {image_file}.")
class
RemoteImageViewer
(
BaseImageViewer
):
"""
View images from OMERO.
"""
#
class RemoteImageViewer(BaseImageViewer):
#
"""View images from OMERO."""
def
__init__
(
self
,
h5file
:
str
,
server_info
:
t
.
Dict
[
str
,
str
],
omero_id
:
int
):
"""
Initialise using a h5file and importing aliby.io.omero.
"""
h5file_path
=
Path
(
h5file
)
super
().
__init__
(
h5file_path
)
with
h5py
.
File
(
h5file_path
,
"
r
"
)
as
f
:
# get image_id from the h5 file
image_id
=
f
.
attrs
.
get
(
"
image_id
"
)
if
image_id
is
None
:
# get image_id from OMERO
with
Dataset
(
omero_id
,
**
server_info
)
as
dataset_om
:
positions
=
dataset_om
.
get_position_ids
()
image_id
=
positions
.
get
(
h5file_path
.
name
.
split
(
"
.
"
)[
0
])
if
image_id
is
None
:
print
(
"
Can
'
t find an image.
"
)
else
:
print
(
f
"
Using image ID
{
image_id
}
.
"
)
self
.
image_id
=
image_id
image
=
OImage
(
image_id
,
**
server_info
)
print
(
"
Connected to OMERO.
"
)
self
.
tiler
=
Tiler
.
from_h5
(
image
,
h5file_path
)
self
.
cells
=
Cells
.
from_source
(
h5file_path
)
# def __init__(
# self, h5file: str, server_info: t.Dict[str, str], omero_id: int
# ):
# """Initialise using a h5file and importing aliby.io.omero."""
# h5file_path = Path(h5file)
# super().__init__(h5file_path)
# with h5py.File(h5file_path, "r") as f:
# # get image_id from the h5 file
# image_id = f.attrs.get("image_id")
# if image_id is None:
# # get image_id from OMERO
# with Dataset(omero_id, **server_info) as dataset_om:
# positions = dataset_om.get_position_ids()
# image_id = positions.get(h5file_path.name.split(".")[0])
# if image_id is None:
# print("Can't find an image.")
# else:
# print(f"Using image ID {image_id}.")
# self.image_id = image_id
# image = OImage(image_id, **server_info)
# print("Connected to OMERO.")
# self.tiler = Tiler.from_h5(image, h5file_path)
# self.cells = Cells.from_source(h5file_path)
def
colormap
(
channel
):
"""
Find default colormap.
"""
if
"
GFP
"
in
channel
:
colormap
=
"
green
"
elif
"
Cherry
"
in
channel
or
"
RFP
"
in
channel
:
colormap
=
"
red
"
else
:
colormap
=
"
gray
"
return
colormap
def
get_files
(
...
...
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