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Swain Lab
wela
Commits
25c73bb5
Commit
25c73bb5
authored
10 months ago
by
pswain
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feature(imageviewer): co-viewing multiple traps
parent
9ff355a4
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src/wela/imageviewer.py
+88
-55
88 additions, 55 deletions
src/wela/imageviewer.py
with
88 additions
and
55 deletions
src/wela/imageviewer.py
+
88
−
55
View file @
25c73bb5
...
@@ -161,7 +161,20 @@ class ImageViewer:
...
@@ -161,7 +161,20 @@ class ImageViewer:
return
tile_dict
return
tile_dict
def
get_data_for_viewing
(
self
,
trap_id
,
tps
,
channels_to_skip
):
def
get_data_for_viewing
(
self
,
trap_id
,
tps
,
channels_to_skip
):
"""
Get images and outlines as multidimensional arrays for Napari.
"""
"""
Get images and outlines as multidimensional arrays for Napari.
Returns
-------
ts_images: array
An array of images ordered by time points, channels, z stack,
x and y values.
ts_labels: array
An array of images of cell outlines ordered by time points,
z stack, x and y values.
channels: list of str
List of channel names.
"""
# get outlines and tiles
# get outlines and tiles
outlines
=
self
.
get_outlines
(
trap_id
,
tps
)
outlines
=
self
.
get_outlines
(
trap_id
,
tps
)
tiles_dict
=
self
.
get_tiles
(
trap_id
,
tps
,
channels_to_skip
)
tiles_dict
=
self
.
get_tiles
(
trap_id
,
tps
,
channels_to_skip
)
...
@@ -183,7 +196,65 @@ class ImageViewer:
...
@@ -183,7 +196,65 @@ class ImageViewer:
ts_labels
[
tp_index
,
0
,
...]
=
outlines
[
tp_index
]
ts_labels
[
tp_index
,
0
,
...]
=
outlines
[
tp_index
]
return
ts_images
,
ts_labels
,
channels
return
ts_images
,
ts_labels
,
channels
def
view
(
self
,
trap_id
,
tpt_end
=
10
,
tpt_start
=
0
,
channels_to_skip
=
None
):
def
concat
(
self
,
arrangement
,
image_dict
,
axis
):
"""
Concat dict of images into one image array.
Following the vertical layout in arrangment.
"""
# concatenate vertically into a list
images_v
=
[
np
.
concatenate
(
[
image_dict
[
trap_id
]
for
trap_id
in
v_stack
],
axis
=
axis
)
for
v_stack
in
arrangement
]
# concatenate horizontally into an array
if
len
(
arrangement
)
>
1
:
images
=
np
.
concatenate
(
[
image_v_stack
for
image_v_stack
in
images_v
],
axis
=
axis
+
1
)
else
:
images
=
images_v
[
0
]
return
images
def
combine_tiles
(
self
,
ts_images_dict
,
ts_labels_dict
,
no_vertical_tiles
):
"""
Combine tiles into one image first vertically then horizontally.
"""
no_tiles
=
len
(
ts_images_dict
)
trap_ids
=
list
(
ts_images_dict
.
keys
())
# find how tiles will be arranged in the concatenated image
if
no_tiles
<
no_vertical_tiles
:
arrangement
=
[
trap_ids
]
else
:
arrangement
=
[
trap_ids
[
i
:
min
(
i
+
no_vertical_tiles
,
no_tiles
)]
for
i
in
range
(
0
,
int
(
np
.
floor
(
no_tiles
/
no_vertical_tiles
)
*
no_vertical_tiles
),
no_vertical_tiles
,
)
]
if
no_tiles
>
np
.
array
(
arrangement
).
size
:
print
(
f
"
Too many tiles:
{
no_tiles
-
np
.
array
(
arrangement
).
size
}
"
"
will be ignored.
"
)
# concatenate images
ts_images
=
self
.
concat
(
arrangement
,
ts_images_dict
,
axis
=
3
)
ts_labels
=
self
.
concat
(
arrangement
,
ts_labels_dict
,
axis
=
2
)
return
ts_images
,
ts_labels
def
view
(
self
,
trap_ids
,
tpt_end
=
10
,
tpt_start
=
0
,
channels_to_skip
=
None
,
no_vertical_tiles
=
3
,
):
"""
"""
Use Napari to view all channels and outlines for a particular trap.
Use Napari to view all channels and outlines for a particular trap.
...
@@ -200,10 +271,22 @@ class ImageViewer:
...
@@ -200,10 +271,22 @@ class ImageViewer:
will be slow.
will be slow.
"""
"""
tps
=
np
.
arange
(
tpt_start
,
tpt_end
+
1
)
tps
=
np
.
arange
(
tpt_start
,
tpt_end
+
1
)
ts_images
,
ts_labels
,
channels
=
self
.
get_data_for_viewing
(
if
isinstance
(
trap_ids
,
int
):
trap_id
,
tps
,
channels_to_skip
trap_ids
=
[
trap_ids
]
ts_images_dict
,
ts_labels_dict
=
{},
{}
for
trap_id
in
trap_ids
:
ts_images_dict
[
trap_id
],
ts_labels_dict
[
trap_id
],
channels
=
(
self
.
get_data_for_viewing
(
trap_id
,
tps
,
channels_to_skip
)
)
# combine tiles
ts_images
,
ts_labels
=
self
.
combine_tiles
(
ts_images_dict
,
ts_labels_dict
,
no_vertical_tiles
)
)
# launch napari
# launch napari
self
.
launch_napari
(
ts_images
,
ts_labels
,
channels
)
def
launch_napari
(
self
,
ts_images
,
ts_labels
,
channels
):
"""
Use Napari to see the images and outlines.
"""
viewer
=
napari
.
Viewer
()
viewer
=
napari
.
Viewer
()
viewer
.
add_image
(
viewer
.
add_image
(
ts_images
[:,
channels
.
index
(
"
Brightfield
"
),
...],
ts_images
[:,
channels
.
index
(
"
Brightfield
"
),
...],
...
@@ -222,57 +305,7 @@ class ImageViewer:
...
@@ -222,57 +305,7 @@ class ImageViewer:
)
)
# class LocalImageViewer(BaseImageViewer):
####
# """
# View images from local files.
# Files are either zarr or organised in directories.
# """
# def __init__(self, h5file: str, image_file: str):
# """Initialise using a h5file and a zarr file of images."""
# h5file_path = Path(h5file)
# image_file_path = Path(image_file)
# if h5file_path.exists() and image_file_path.exists():
# super().__init__(h5file_path)
# with dispatch_image(image_file_path)(image_file_path) as image:
# self.tiler = Tiler.from_h5(image, h5file_path)
# self.cells = Cells.from_source(h5file_path)
# else:
# if not h5file_path.exists():
# print(f" Trouble loading {h5file}.")
# if not image_file_path.exists():
# print(f" Trouble loading {image_file}.")
# class RemoteImageViewer(BaseImageViewer):
# """View images from OMERO."""
# def __init__(
# self, h5file: str, server_info: t.Dict[str, str], omero_id: int
# ):
# """Initialise using a h5file and importing aliby.io.omero."""
# h5file_path = Path(h5file)
# super().__init__(h5file_path)
# with h5py.File(h5file_path, "r") as f:
# # get image_id from the h5 file
# image_id = f.attrs.get("image_id")
# if image_id is None:
# # get image_id from OMERO
# with Dataset(omero_id, **server_info) as dataset_om:
# positions = dataset_om.get_position_ids()
# image_id = positions.get(h5file_path.name.split(".")[0])
# if image_id is None:
# print("Can't find an image.")
# else:
# print(f"Using image ID {image_id}.")
# self.image_id = image_id
# image = OImage(image_id, **server_info)
# print("Connected to OMERO.")
# self.tiler = Tiler.from_h5(image, h5file_path)
# self.cells = Cells.from_source(h5file_path)
def
colormap
(
channel
):
def
colormap
(
channel
):
"""
Find default colormap.
"""
"""
Find default colormap.
"""
if
"
GFP
"
in
channel
:
if
"
GFP
"
in
channel
:
...
...
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